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What is science?
A process of gathering information about the observable world and organizing the information into testable theories/laws/models. 
Scientia
Latin for knowledge 
What is the scientific method?
1) Observation and Description of a Phenomenon 2) Models- Generalizations and Explanations 3) Predictive Statements 4)Tests- Experiments 
Give an example of an observation
Maggots live in meat 
Give an example of a model
Decaying meat produces Maggots 
What is a Predictive Statement?
It must be testable. EX- If maggots are spontaneously generated by rotting meat, then rotting meat in isolation from all other influence will produce maggots 
What must tests and experiments be?
They must be controlled and replicated 
Who preformed what is considered to be the first scientific experiment that tested the concept of spontaneous generation of complex life-forms?
Francesco Redi(1668) 
The null and alternative hypotheses should cover _____ possible results that you may get at the end of your experiment.
ALL 
What does a null hypothesis state?
There will be NO difference or no change between the treatment and control in your experiment 
What does a alternative hypothesis state?
There WILL be a difference / change between the treatment and the control. 
What is a hypothesis?
Prediction or explanation that has not been tested 
What is a theory?
Prediction or explanation that has been repeatedly tested (by many people, in many ways, and for a long time) and is strongly supported. 
What is a law?
Theory tested many times and result same every time. 
What three organisms did we work with the first week of lab?
Isopods Termites Copepods 
What is Kinesis? Give an example
Movements irrespective of the source of stimulus. Eg. movement of woodlice is proportional to the humidity of the environment 
What is Taxis? Give an example
Orientation of animal body is relative to stimulus source. Eg. Plants grow towards the direction of sunlight. 
________ will only state whether we will or will not see a difference between 2 groups.
Hypothesis 
What is a prediction?
This states in what directions we will see that difference. Predictions are more specific then hypothesis, and more interesting. 
What are things needed for experimental design?
1) Number of Variables to be manipulated (Treatment) 2) Control Group 3) Replicates 4) Scale of Measurements 5) Criteria for supporting/rejecting your hypothesis 
How many variables will we usually manipulate? What is this group called?
We will manipulate one. The group in which the variable is manipulated is called the experimental group or treatment group. 
What is a control group?
Standard to which we compare our results- so we can tell if the treatment has an effect. Attempt to equalize all the external conditions. 
What are replicates?
Repetition increases the reliability and accuracy of your results. Minimum of 3 replicates. 
What is the scale of measurements?
What are you measuring? Better measure things in a small scale and then lump data into groups than to use a scale so big that you can miss important differences. Need a reasonable time frame. Metric Units. 
Criteria for supporting/rejecting your hypothesis
- How big of a difference you need to see? - Descriptive STATISTICS to characterize the population of data points that you have collected 
What is the MEAN?
Average of a series of data points. 
What is the STANDARD DEVIATION?
Describes how much the data spreads from the mean. 
How would you obtain the mean of something?
Add up all the data of that column and divide by how many replicates. 
What all do you need to label?
Title, Both X and Y axis. 
Graphs show _____.
Trends
Tables show exact ____.
Values
What is the independent variable?
What you manipulated and the x-axis 
What is the dependent variable?
What you measured and the y-axis. 
When you label both axis, include the _____.
Units
What are descriptive statistics?
They describe the population of data points collected. 
What is a sample vs. a population?
A sample is a small subset representative of a population. 
The that standard deviation to plot ____ ____.
Error Bars 
If the error bars overlap is there a statistically significant difference?
NO 
If the error bars do not overlap is there statistically significant difference?
YES 
What are the characteristics of life?
Living things demonstrate all these qualities- 1) Energy Transformation 2) Complexity 3) Growth/Reproduction 4) Response to Stimuli 5) Capacity to Evolve 
What is Energy Transformation?
Gas Exchange or converting energy from one form to another form. 
What is an example of Energy Transformation?
Cellular Respiration, Photosynthesis, Breathing, Eating 
What is complexity?
A high level of organization. Complex ORGANIC MOLECULES, cellular structures. 
What are some forms of complexity?
DNA, Glucose, Carbon Molecule 
What does it mean to reproduce or grow?
This means to develop. 
What is a response to stimuli?
Such as movement or seasonality 
What is evolving?
Occurs on a different time scale than the other characteristics. 
Life is ____ defined by any one characteristic.
NOT 
In the what is life lab what were we trying to determine?
Was life ever present on Mars or if it is currently present on Mars. 
What three samples did we use?
Sterile Martian Soil Native Martian Soil Sterile Sand 
What does the Benedict Test for?
Presence of reducing sugars. Shows a level of high organization. 
What does the Biuret Test for?
Presence of peptide bonds. Shows a level of high organization. 
What does microscopy test for?
Presence of complex structures. Shows a high level of organization. 
What does pH test for?
CO2 Production. Shows energy transformation and gas exchange. 
What does Fermentation test for?
CO2 Production. Shows energy transformation and gas exchange. 
What does Agar plating test for?
Presence of new individuals. Shows reproduction. 
When doing the Benedict's Test what will happen if there is a presence of sugars?
The solution will turn from blue to a red/orange 
When doing the Biuret's Test what will happen if these is a presence of peptide bonds?
The solution will turn from blue to purple. 
When preforming the Fermentation test what would indicate gas formation?
Bubbles at the top of the tube 
What is evolution?
A change in the genetic make-up of a population over time. 
What is small scale evoultion?
Changes in gene frequency in a population from one generation to the next. 
What is large scale evoultion?
Descent of different species from a common ancestor over many generations. 
What is the genetic make-up?
Frequency of Alleles 
A single gene in a population may have several alternate forms—or ____—which account for variation between the ____ of the organisms.
Alleles Phenotypes 
What is an allele?
For example: There is a gene for color of eyes in humans, but there are many alleles (versions) of the gene 
_____ ______ have two alleles for each trait.
Diploid Organisms 
Normally one is ______ and the other is ______.
Dominant Recessive 
Caused by a recessive allele.
Cystic Fibrosis 
What is a population?
Individuals of the same species that live in a particular place at a specific time. 
Individuals ___ ___ evolve.
DO NOT 
The genetic make-up of an individual is ____ and will ___ change over time.
Fixed Not 
Evolution occurs over many _______.
Generations
How does evolution happen?
Accumulation of changes in the genetic material. 
evolution requires _____ and _____.
Reproduction and Inheritance 
How does the genetic make-up (gene frequencies) of a population change?
Mutations Gene Flow Sexual Reproduction 
What are Mutations?
Changes in DNA. 
A ____ mutation can have a large effect, but in many cases, evolutionary change is based on the accumulation of many _____.
Single Mutations This is RARE 
What is Gene flow?
This is any movement of genes from one population to another and is an important source of genetic variation. This is COMMON. 
What is sexual reproduction?
This can introduce new gene combinations into a population. This is COMMON. 
What drives evolution?
Natural Selection 
What is natural selection?
Some individuals produce more offspring or their offspring survive better than others because of some characteristic/trait of the individual. 
What does Natural Selection Require?
Variation in a population. 
What are the steps of Natural Selection?
1) There is variation in traits 2) There is differential survival/reproduction 3) There is heredity 4) End Result- More advantageous trait becomes more common in the population 
What is the source of variation?
Genetic Mutation and Recombination Environmental- soil quality, weather, nutrients that are available 
What are the sources of genetic variation?
Mutation Sexual Reproduction 
What happens with mutations?
Only true source of new alleles Substitutions, insertions, deletions 
What happens with sexual reproduction?
Alleles are mixed up in different combinations Contributes the most to variation 
What is asexual reproduction?
-Type of reproduction in which there is no fusion of gametes and in which the genetic make up of parents and of offspring is usually identical (mitosis). -Clones of parents 
Sea Anemone reproduce by ____.
Fission 
Sea Sponges reproduce by ______.
Fragmentation 
Why is asexual reproduction beneficial?
The organism does not have to find a mate and they also reproduce rapidly. 
Does asexual reproduction offer variation?
NO 
What is sexual reproduction?
-Type of reproduction in which two gametes (meiosis) fuse to form a zygote. -There is exchange of genetic material, recombination 
What is an organism risking for sexual reproduction?
This is costly for the organism but is beneficial in long term. 
What is the power in sexual reproduction?
Power of sexual reproduction is that you can get a trait or characteristic not present in the parents, which creates variation within the population. 
What are Sordaria?
Ascomycete Fungus 
During the process of sexual reproduction for the Sordaria the fungus forms a structure called ____.
Ascus 
Each ascus contains 8 ______.
Ascuspores 
What is fitness?
The ability of an individual to pass genes on to future generations. 
A fit individual leaves many _____ in the next generation (passes on his/her heritable ______)
Offspring Phenotype 
Has nothing to do directly with the ____ ______ of the individual.
Physical Health 
An individual adapted to the _____ environment is likely to produce more offspring than an individual who is not as well adapted (but chance also plays a role).
Local 
The fitness of different individuals with the same ______ is not necessarily equal but depends on the environment in which the individuals live.
Genotype 
What is natural selection?
Tendency of organisms that possess FAVORABLE ADAPTATIONS to their ENVIRONMENT to SURVIVE and PASS THEIR GENES to the next generation through reproduction. 
_____ _____ occurs when natural selection results in a CHANGE IN THE ALLELE FREQUENCY of a population.
Evolution 
____ ______tends to result in the removal of the least fit organism from the population.
Natural Selection 
This depends on the ______.
Environment 
How does the local environment impact the fitness of an individual?
Defines which phenotypes have the best characteristics (traits) to survive and reproduce. 
Can an individual be fit in one environment but not another?
YES 
What is co-evolution?
When species effect the way each other species evolves. Such as a humming bird and their host flower. 
The environment is "selecting" for or against individuals from a population though ____ _____.
Natural Selection 
Selection ACTS on the ______.
Phenotype 
But its EFFECTS are fixed only for _____ _____ of a population.
Heritable Traits 
Observed quality of an organism (e.g. morphology, behavior, development).
Phenotype 
Genetic make-up of an organism that may or may not be expressed (show up in the phenotype)
Genotype 
Phenotype=
Enviroment+Genotype 
Most common strain of flies. Can fly, smell, and fit into small spaces.
Wild Type 
Lack Wing, they cannot fly.
Apterous 
Wings always held out; they cannot move through very small spaces.
Held out 
Their antennae develop into legs, they have a reduced sense of smell.
Antennapedia 
What is homeostasis?
A property of a living organism, that regulates its internal environment so as to maintain a stable, constant condition. 
Homeostasis has survival value because it means an animal can adapt to a _____ ______.
Changing Environment 
What does an organism need to regulate?
Ion levels (Na+, K+, O2) Nutrients: Sugar levels Water levels TEMPERATURE 
How do organisms control their temperature?
2 categories of organisms based on how they regulate their temperature 
What is a homeotherm?
-Capable of maintaining its body temperature within a narrow range. -Temperature is usually above that of its surroundings despite large variations in environmental temperature. -Mammals 
What is a Poikilotherm?
-Body temperature fluctuates with that of the environment. -Cannot maintain a constant body temperature, affected by environmental changes -Reptiles 
Animals use ______ adaptations to regulate body temperature.
Behavioral -On a cool morning a snake might move into a sunny area to absorb heat from the sun. -In a large lake, a fish might move into deeper, cooler water when surface waters get too warm. 
What are some sources of exchanging heat?
Surfaces → Conduction Air → Convection Sun → Radiation Evaporation 
What aspects/structures of an organism may impact the gain and loss of heat?
Size, Shape, Legs, Color, Sweat, Location 
How does size effect heat?
bigger organisms change temperature more slowly 
How does shape effect heat?
increase/decrease surface area for the conduction of heat 
How do legs effect heat?
keeping the body off the surface (conduction) 
How does color effect heat?
radiation of light/sun 
How does sweat effect heat?
lose heat through evaporation 
How does location effect heat?
move to minimize/ maximize conduction, convection or radiation 
Size
Hot (heat pads) or cold (ice box) surface - CONDUCTION 
Shape
Wind (fan) - CONVECTION 
Legs
Sun (lamps) - RADIATION 
Color
Shade (sand box) - RADIATION 
Sweat
High humidity (spray bottle): EVAPORATION 
What is the function of the cell membrane?
protect, separate, regulate 
Molecules have to pass into and out of a cell through the
Membrane 
It allows cells to control their internal ______ environment and the availability of important materials.
Chemical 
What is the structure of the cell membrane?
Phospholipid Bilayer 
Heads are _____ which means water ____.
Hydrophilic , loving 
Tails are _____ which means water ______.
Hydrophobic, Fearing 
What can automatically pass through the cell membrane?
O2 , CO2 H2O and other small, or non-polar molecules 
What cannot automatically pass through the cell membrane?
Glucose and other large, polar, water- soluble molecules; ions (H+, Na+, K+, CA++, CI-) 
What are two types of passive transport?
Diffusion Osmosis 
What is Diffusion?
Spontaneous NET movement of particles from an area of high solute concentration to an area of low concentration in a given volume of fluid (liquid or gas) 
What is Osmosis
Spontaneous NET movement of water through a cell wall or membrane or any semi-permeable barrier from an area of low solute concentration to an area with high solute concentration 
What is active transport?
An energy-requiring process that moves materials across a cell membrane. 
Molecules are forced through a channel/carrier protein with the aid of ______ in the form of ATP.
Energy 
The cell uses active transport in three situations. What are they?
1-When a particle is going from low to high concentration (i.e. against a concentration gradient) 2- When particles need help entering the membrane because they are selectively impermeable 3- When very large particles enter and exit the cell 
What is Phagocytosis?
Cellular process of engulfing solid particles by the cell membrane to form an internal phagosome, or "food vacuole". 
Particle is in the cell but considered ______.
Separate 
What is a hyper tonic solution?
contains a high solute concentration relative to another solution. 
What is a hypotonic solution?
contains a low solute concentration relative to another solution. 
What is an isotonic solution?
contains an equal solute concentration relative to another solution. 
What determines whether a material can move across the cell membrane?
Size, Shape, Polarity 
Particles that move fast usually have:
Small size No charge- (membranes are composed of non-polar phosopholipids) *Water molecules can move freely across the cell membrane because they are small (and despite the fact they are polar) 
What is osmotic concentration?
concentration of solutes (dissolved substances) in the water 
When the osmotic concentrations of two regions differ, water will flow from the area of ____ solute concentration to the area of ____ concentration.
Low High 
What is lyse?
When more and more water moves into the cell and eventually burst. 
How do you determine the osmotic concentration from a graph?
When putting something straight on the graph like a pen it is where the pen hits the data line. 
What happens if we put Red Blood Cells (RBCs) in an isotonic solution?
Technically Nothing 
What if the isotonic solution has a solute that can diffuse through the cell membrane?
Then the solute will diffuse across the cell membrane 
What happens to the solute concentration of the cell as more and more solute moves into the cell?
The inside of the cell becomes hypertonic (high solute concentration) compared to the outside solution 
Now that the inside of the cell is hypertonic to the extracellular solution, in what direction will water move?
Water will move from the extracellular solution (low solute concentration) to the inside of the cell (high solute concentration). 
What question did we ask for one of the RBC's lab?
How does the time to hemolysis for RBCs differ in solutions of glycols of different molecular weights? 
What was the other question?
How does the time to hemolysis differ for RBCs in solutions of propanes with different molecular charges (polarity)? 
Population size is ____. What does this mean?
Dynamic- it is always changing over time. 
What is population growth rate?
Rate at which the number of individuals in a population increase per unit time 
What is the equation for population growth rate?
(Population at end of period - Population at beginning of period)/Population at beginning of period 
What are two of the most important mathematical models of population growth?
Exponential Growth Logistic Growth 
What is the importance of the population growth models?
-How long will it take for a population to reach a given size -What will population size be after n years (or generations)? -What happens when there are limited resources? *Go look at these graphs 
What is the bio-geochemical cycle?
Biogeochemical cycle by which carbon is exchanged between the biosphere, geosphere, hydrosphere, and atmosphere of the Earth. 
What are the sources of Carbon?
CO2 atmosphere C6H12O6 organisms H2CO3 ocean CH4 animal matter CaCO3 organisms 
The most important feature of the carbon cycle is the circulation of carbon from the _____ environment into living things (____) and back into the ____ enviroment.
Abiotic Biotic Abiotic 
Photosynthesis:
Abiotic - biotic environment 
Cellular Respiration:
Biotic - abiotic environment 
How is carbon released into the atmosphere?
Cellular Respiration Decay Burning Fossil Fuels At the Surface of the Oceans 
What does Photosynthesis require and produce?
CO2 O2 
What does cellular respiration require and produce?
O2 CO2 
What is the equation for Photosynthesis?
6H2O + 6CO2 ----------> C6H12O6 (sugar) + 6O2 
What is the equation for Cellular Respiration?
C6H12O6 (sugar) + 6O2 (oxygen) ----------> 6H2O (water) + 6CO2 + ATP (energy)

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