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Gel activity sheet MCB 251 30 pts 1 Use the following plasmid map to calculate the expected DNA fragment sizes for each restriction digestion reaction List the sizes from smallest to largest Include units 15 pts A NheI B BamHI and PciI C EcoRV and DraIII D ClaI XhoI and XmaI E PvuII and SalI 2 Draw the bands corresponding to the DNA fragments from the above restriction digestion reactions 15 pts Lanes B E A D C 10 kb 8 kb 6 kb 5 kb 4 kb 3 kb 2 kb 1 5 kb 1 kb 0 5 kb a

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