Domenica DiStasio Wednesday 9 AMFigure 1: India Ink Phagocytosis in Tetrahymena. The graph above shows the results of the effect on India Ink Phagocytosis in Tetrahymena. The results were observed under a microscope at 10x magnification. We looked at how the Tetrahymena grew food vacuoles in response to the India Ink. We measured this in ten-minute increments ranging from 0 to 40 minutes. The Tetrahymena were fixed with glutaraldehyde to stop the cells from eating the India Ink so we could get an accurate reading of the number of food vacuoles present in the cells.From this experiment, we were able to collect data on how fast or slow the Tetrahymena organism was able to absorb the India Ink and create food vacuoles. We did this by adding the Tetrahymena to a solution of India Ink. We then placed this solution into a glutaraldehyde solution in ten-minute increments. Each sample of Tetrahymena was placed in five separate tubes, each labeled from zero to forty seconds. The glutaraldehyde kills the cells without destroying the tissues so that we are able to observe these cells under a microscope. We did this at 10x magnification and looked at how many food vacuoles were present. From the data, we can conclude that as the time went on, more food vacuoles formed, which therefore means that more and more of the India Ink was being eaten by the Tetrahymena. We analyzed the data by getting the mean and standard deviation of the cells that were counted after the ten to forty minutes. We conclude from figure 1, that after 0 minutes the Tertrahymena should have close to 0 food vacuoles because that have not yet been able to eat the India Ink. The mean for time 0 was 1.4, which was due to our error in not being fast enough in moving that solution into the glutaraldehyde. At the end of the forty minutes, the Tetrahymena cells had grown an average of 18 food vacuoles, and they were still
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