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PCC BI 232 - Sylvania Laboratory Survival Guide

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Anatomy and Physiology IIFall 2007 UpdateLab TopicPCC LabGuide Page1 Safety GuidelinesObjective: Learn to actively search for useful resources on the World Wide Web and to engage you in critical analysis as you judge the credibility of what you find.I. AuthorityII. AccuracyII. ObjectivityIV. CurrencyV. CoverageMartini Chapter 13, pages 423-437Gross AnatomyCervical Plexus C1-C5Martini Chapter 13, pages 439-4461.1 Receptor1.2 Sensory neuron1.3 Reflex center1.4 Motor neuron1.5 EffectorMeningesVentriclesBrainstemCerebrumMartini Chapter 18Martini Chapter 19ReticulocyteNeutrophilEosinophilBasophilMonocyteLymphocyteMaking a Blood Smear using Wright’s StainHemoglobin ConcentrationsMartini Chapter 20Martini Chapter 20, pages 687-688Martini Chapter 21ArteriesVeinsBiology 232Anatomy and Physiology IISylvania LaboratorySurvival GuideLab Objectives and Worksheets to accompanyFundamentals of Human Anatomy and Physiology, By Frederic Martini, 7th EditionFall 2007 UpdateBI 232 Lab Survival Guide http://spot.pcc.edu/anatomy/lab.htm Fall 20070Table of ContentsLab Topic MartiniPCC LabGuide Page1 Safety Guidelines ---- 2 Disposal Guidelines---- 3 Website Review Directions---- 413. Spinal Cord Ch 13 (pages 423-437) 514. Reflexes Ch 13 (pages 439-446) 1515. Brain Chapter 14 2116. Olfaction & Taste Ch 17 (pages 550-554) 3317. Vision Ch 17 (pages 554-572) 3718. Hearing Ch 17 (pages 573-586) 4619. Cranial Nerves Ch 14 (pages 480-490) 5120. General Senses Ch 15 (pages 495-502) 5921. Endocrine & Glucometer Chapter 18 6522. Blood Chapter 19 7123. Cardiac Anatomy Chapter 20 8124. EKG Ch 20 (pages 687-688) 8925. Blood Vessels & Circulation Chapter 21 10226. Blood Pressure & Pulse ---- 119 Evaluation Form---- 121Lab PowerPoint slides can be viewed at:http://spot.pcc.edu/anatomy/lab.htmBI 232 Lab Survival Guide http://spot.pcc.edu/anatomy/lab.htm Fall 20071PCC-Sylvania BI 232 Laboratory Supplement1. Upon entering the laboratory, please locate the exits, fire extinguisher, eyewash station, and clean up materials for chemical spills. Your instructor will demonstrate the location of fire blanket, safety kit, andshowers.2. Read the general laboratory directions and any objectives before coming to lab.3. Food and drink, including water, are prohibited in laboratory. This is per Federal laboratory guidelines and per College Safety Policy. Do not chew gum, use tobacco products of any kind, store food or apply cosmetics in the laboratory. No drink containers of any kind may be on the benches.4. Please keep all personal materials off the working area. Store backpacks and purses at the rear of the laboratory, not beside or under benches. Some laboratory spaces have shelving in rear for this purpose.5. For your safety, please restrain long hair, loose fitting clothing and dangling jewelry. Hair ties are available, ask your instructor. Hats and bare midriffs are not acceptable in the laboratory. Shoes, not sandals, must be worn at all times in laboratory. You may wear a laboratory apron or lab coat if you desire, but it is not required.6. We do not wish to invade your privacy, but for your safety if you are pregnant, taking immunosuppressive drugs or who have any other medical conditions (e.g. diabetes, immunological defect) that might necessitate special precautions in the laboratory must inform the instructor immediately. If you know you have an allergy to latex or chemicals, please inform instructor.7. Decontaminate work surfaces at the beginning of every lab period using Amphyl solution. Decontaminate bench following any practical quiz, when given, and after labs involving the dissection of preserved material.8. Use safety goggles in all experiments in which solutions or chemicals are heated or when instructed to do so. Never leave heat sources unattended: hot plates or Bunsen burners.9. Wear disposable gloves when handling blood and other body fluids or when touching items or surfaces soiled with blood or other body fluids such as saliva and urine. (NOTE: cover open cuts or scrapes with a sterile bandage before donning gloves.) Wash your hands immediately after removing gloves.10. Keep all liquids away from the edge of the lab bench to avoid spills. Immediately notify your instructor of any spills. Keep test tubes in racks provided, except when necessary to transfer to water baths or hot plate. You will be advised of the proper clean-up procedures for any spill.11. Report all chemical or liquid spills and all accidents, such as cuts or burns, no matter how minor, to the instructor immediately.12. Use mechanical pipetting devices only. Mouth pipetting is prohibited.Students who do not comply with these safety guidelines will be excluded from the LaboratoryBI 232 Lab Survival Guide http://spot.pcc.edu/anatomy/lab.htm Fall 20072Safe Disposal of Contaminated Materials• Place disposable materials such as gloves, mouth pieces, swabs, toothpicks and paper towels that have come into contact with blood or other body fluids into a disposable Autoclave bag for decontamination by autoclaving.This bucket is not for general trash.• Place glassware contaminated with blood and other body fluids directly into a labeled bucket of 10% bleach solution. ONLY glass or plastic-ware is to be placed in this bucket, not trash.• Sharp’s container is for used lancets only. It is bright red. When using disposable lancets do not replace their covers.1. Properly label glassware and slides, using china markers provided.2. Wear disposable gloves when handling blood and other body fluids or when touching items or surfaces soiledwith blood or other body fluids such as saliva and urine. (NOTE: cover open cuts or scrapes with a sterile bandage before donning gloves.) Wash your hands immediately after removing gloves.3. Wear disposable gloves when handling or dissecting specimens fixed with formaldehyde or stored in Carosafe/Wardsafe.4. Wear disposable gloves when handling chemicals denoted as hazardous or carcinogenic by your instructor. Read labels on dropper bottles provided for an experiment, they will indicate the need for gloves or goggles, etc. Upon request, detailed written information is available on every chemical used (MSDS). Ask your instructor.5. No pen or pencil is to be used at any time on any model or bone. The bones are fragile, hard to replace and used by hundreds of students every year. To protect them and keep them in the best


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