Biology 232 Anatomy and Physiology II Sylvania Laboratory Survival Guide Lab Objectives and Worksheets to accompany Fundamentals of Human Anatomy and Physiology, By Frederic Martini, 7th Edition Fall 2007 UpdateBI 232 Lab Survival Guide http://spot.pcc.edu/anatomy/lab.htm Fall 2007 1Table of Contents Lab Topic Martini PCC Lab Guide Page  Safety Guidelines ---- 2  Disposal Guidelines ---- 3  Website Review Directions ---- 4 13. Spinal Cord Ch 13 (pages 423-437) 5 14. Reflexes Ch 13 (pages 439-446) 15 15. Brain Chapter 14 21 16. Olfaction & Taste Ch 17 (pages 550-554) 33 17. Vision Ch 17 (pages 554-572) 37 18. Hearing Ch 17 (pages 573-586) 46 19. Cranial Nerves Ch 14 (pages 480-490) 51 20. General Senses Ch 15 (pages 495-502) 59 21. Endocrine & Glucometer Chapter 18 65 22. Blood Chapter 19 71 23. Cardiac Anatomy Chapter 20 81 24. EKG Ch 20 (pages 687-688) 89 25. Blood Vessels & Circulation Chapter 21 102 26. Blood Pressure & Pulse ---- 119  Evaluation Form ---- 121 Lab PowerPoint slides can be viewed at: http://spot.pcc.edu/anatomy/lab.htmBI 232 Lab Survival Guide http://spot.pcc.edu/anatomy/lab.htm Fall 2007 2PCC-Sylvania BI 232 Laboratory Supplement 1. Upon entering the laboratory, please locate the exits, fire extinguisher, eyewash station, and clean up materials for chemical spills. Your instructor will demonstrate the location of fire blanket, safety kit, and showers. 2. Read the general laboratory directions and any objectives before coming to lab. 3. Food and drink, including water, are prohibited in laboratory. This is per Federal laboratory guidelines and per College Safety Policy. Do not chew gum, use tobacco products of any kind, store food or apply cosmetics in the laboratory. No drink containers of any kind may be on the benches. 4. Please keep all personal materials off the working area. Store backpacks and purses at the rear of the laboratory, not beside or under benches. Some laboratory spaces have shelving in rear for this purpose. 5. For your safety, please restrain long hair, loose fitting clothing and dangling jewelry. Hair ties are available, ask your instructor. Hats and bare midriffs are not acceptable in the laboratory. Shoes, not sandals, must be worn at all times in laboratory. You may wear a laboratory apron or lab coat if you desire, but it is not required. 6. We do not wish to invade your privacy, but for your safety if you are pregnant, taking immunosuppressive drugs or who have any other medical conditions (e.g. diabetes, immunological defect) that might necessitate special precautions in the laboratory must inform the instructor immediately. If you know you have an allergy to latex or chemicals, please inform instructor. 7. Decontaminate work surfaces at the beginning of every lab period using Amphyl solution. Decontaminate bench following any practical quiz, when given, and after labs involving the dissection of preserved material. 8. Use safety goggles in all experiments in which solutions or chemicals are heated or when instructed to do so. Never leave heat sources unattended: hot plates or Bunsen burners. 9. Wear disposable gloves when handling blood and other body fluids or when touching items or surfaces soiled with blood or other body fluids such as saliva and urine. (NOTE: cover open cuts or scrapes with a sterile bandage before donning gloves.) Wash your hands immediately after removing gloves. 10. Keep all liquids away from the edge of the lab bench to avoid spills. Immediately notify your instructor of any spills. Keep test tubes in racks provided, except when necessary to transfer to water baths or hot plate. You will be advised of the proper clean-up procedures for any spill. 11. Report all chemical or liquid spills and all accidents, such as cuts or burns, no matter how minor, to the instructor immediately. 12. Use mechanical pipetting devices only. Mouth pipetting is prohibited. Students who do not comply with these safety guidelines will be excluded from the LaboratoryBI 232 Lab Survival Guide http://spot.pcc.edu/anatomy/lab.htm Fall 2007 3Safe Disposal of Contaminated Materials • Place disposable materials such as gloves, mouth pieces, swabs, toothpicks and paper towels that have come into contact with blood or other body fluids into a disposable Autoclave bag for decontamination by autoclaving. This bucket is not for general trash. • Place glassware contaminated with blood and other body fluids directly into a labeled bucket of 10% bleach solution. ONLY glass or plastic-ware is to be placed in this bucket, not trash. • Sharp’s container is for used lancets only. It is bright red. When using disposable lancets do not replace their covers. 1. Properly label glassware and slides, using china markers provided. 2. Wear disposable gloves when handling blood and other body fluids or when touching items or surfaces soiled with blood or other body fluids such as saliva and urine. (NOTE: cover open cuts or scrapes with a sterile bandage before donning gloves.) Wash your hands immediately after removing gloves. 3. Wear disposable gloves when handling or dissecting specimens fixed with formaldehyde or stored in Carosafe/Wardsafe. 4. Wear disposable gloves when handling chemicals denoted as hazardous or carcinogenic by your instructor. Read labels on dropper bottles provided for an experiment, they will indicate the need for gloves or goggles, etc. Upon request, detailed written information is available on every chemical used (MSDS). Ask your instructor. 5. No pen or pencil is to be used at any time on any model or bone. The bones are fragile, hard to replace and used by hundreds of students every year. To protect them and keep them in the best condition, please use pipe cleaners and probes provided instead of a writing instrument. a. Probes may be used on models as well. The bones are very difficult and costly to replace, as are the models and may take a long time to replace. 6. At the end of an experiment: a. Clean glassware and place where designated. Remove china marker labels at this time. b. Return solutions & chemicals to designated area. Do not put solutions or chemicals in cupboards! 7. You cannot work alone or unsupervised in the laboratory. 8. Microscopes should be cleaned before returning to numbered cabinet. Be sure objectives are