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MIT 7 03 - Exams

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ExamsFall 1998O.t,%t:b©(7.03 Exam 1 lCL_dName:Section: TA:Exam starts at 11:05 and ends at 11:55There are six pages including this cover pagePlease write your name on each page.Please...• Look over the entire exam so you don't spend too much timeon hard questions leaving easy questions unanswered.° Check your answers to make sure that they make sense.° To help us give partial credit, show your work andany assumptions that you make.Question 1 30 pointsQuestion 2 35 pointsQuestion 3 35 pointsName:1. The genes for two human autosomal dominant traits are 10 cM apart (as determined bymeiosis in females). In the following pedigrees the traits are indicated as follows:Q = individual with trait 1 Q = individual with trait 2 G = individual with trait 1 and trait 2(a 15 pts.) For each of the pedigrees shown below, calculate the probability that theindividual designated by "?" will have either dominant trait 1, dominant trait 2, or both traits.'___t_.+4-4-',.21_ --rProbabilityDominant trait 1 only I/4-Dominant trait 2 only i/4-Both trait 1 and trait 2 1/_(b 15 pts.)+2.ProbabilityDominant trait 1 only °Dominant trait 2 only (° c1")4_/_') = •4-£Both trait 1 and trait 2 (- i")( _tZ'_ = • O_" _-_Name:2. Wild-type Drosophila have red eyes, and white eyes are the result of an X-linkedrecessive mutation. A new recessive mutation that gives apricot colored eyes is isolated. Afemale from a true-breeding apricot strain is crossed to a mate from a true-breeding whitestrain, and all of the F1 females have pale-apricot eyes and all of the males have apricoteyes.(a 5 pts.) Are the white and apricot mutations in the same gene or in different genes?Explain your answer._la dj_nc . (qT_ _vD q_n_s do _o_ compidm_r_t-.')_f¢ _qStj &_#_Gam_tcnl_nt,-fhOG. _.qaf roqnv rddlA collection of F1 females from the cross described above (all with pale-apricot eyes) arecrossed to males from a true breeding white eyed strain and 1000 progeny are examined.Among these progeny, 6 flies have normal red eyes.(b 5 pts.) What is the measured distance between the white and apricot mutations in cM?Sadra_r¢_ Z rd:_omt;_tnt C]A¢(;_S" rc_JcclCS ; r_Jottb_d__ttC_nt.T0t_l -racomt4n_r,t_ ?_LL_]b(¢t&n_ Ct_l_ : 2(0;) x too-= l.'zclVt.ioooA new recessive eye color mutation known as peach is isolated. A female from a true-breeding peach strain is crossed to a male from a true-breeding white strain, and all of the F1females have normal red eyes and all of the males have peach eyes.(c 5 pts.) Is the peach mutation on an autosome or on the X-chromosome? Explain youranswer._a mcd_s)(d 5 pts.) Are the white and peach mutations in the same gene or in different genes? Explainyour answer.t._ _ reA-eqed _n,q_le.sName:The recessive mutation for crossveiniess wings lies on the X-chromosome. A female from atrue-breeding strain with apricot eyes and crossveintess wings is crossed to a male from atrue breeding strain with white eyes and normal wings. As expected, all of the F1 females ...._,from this cross have pale apricot eyes and norrnal wings. A large collection of these F1females are crossed to wild-type males and 10,000 male progeny are examined. Theobserved phenotypes are as follows:Phenotype Numbernormal wings white eyes 4,418qIcv wings apricoteyes 4,330 ]normal wings apricot eyes 610"_ 4_iOz_'_dO_(3SS©dOfcv wings white eyes 590J ___weoR W. _tq._ C:v'normal wings red eyes 2 "_ u4ObLbJoC(b_£ O'JC_'_cv wings red eyes 50 _ _ £ilq_.£ C_O<_SeVdfbd_,'dd.d:tq(e 15 pts.) Draw a genetic map showing the relative order and distances in cM between thecrossveinless, apricot and white mutations.i,o_- {-z.04z. "_ 4----q-_ 4 -_-9 w CVa_p-w c,tJ,_t_aec: 2 tz:) + 2-ts-o')_'°°_._oq- _oo- _--- _ -_ i.o4- cN\_,0t _ iOlbOO>c{oo .___11.o4- clV_io_oOoName:3. (a5 pts.) In a yeast cross the segregation of two mutationsare followed. Amo.._j4he-3_ A]i --,(b 5 pts.) In another cross involving two different mutations, PD = 40, T = 8, NPD = 2.Give as much information as you Can about the chromosomal location of these two mutations.20c&@You have isolated two new yeast mutations, big1 is a mutation that produces colonies thatare larger than normal, and .smll is a mutation that produces colonies that are smaller thannormal.e) = wild-type colony= big1 colony@ =smtl colonyA big1 mutant is mated to a smll mutant of the opposite mating type, the resulting diploid issporulated and 12 tetrads are dissected. The tetrad dissection plate looks like this:o o _@® o o@e o@_o@¢@o o o ® o .@@ 1_ @ ® ® _) @ @ @ @ ® @@@_ _@¢ e _ o@@oName:(c 5 pts.) What is thephenotypeof a big1 stall doublemutant?(Doesit looklike wild-type,a big1 mutant,or a stall mutant?)4- 4--(d 10 pts.) Among the t2 tetrads shown above, how many tetrads are there of each type?PD=_ T= _7 NPD=2--What is the linkagerelationshipbetweenbig1 andstall? Givedistancesin cM ifapplicable. L_Ur_V-dd (_b_ _')7.03 E×am 2Name:Section: TA:Exam starts at 11:05 and ends at 11:55There are seven pages including this cover pagePlease write your name on each page.Please...o Look over the entire exam so you don't spend too much timeon hard questions leaving easy questions unanswered.o Check your answers to make sure that they make sense.• To helpus give partialcredit,showyour work andany assumptions that you make.Question 1 35 pointsQuestion 2 30 pointsQuestion 3 35 points• :? i_l _1 J[l_- 1_!11h e You have isolated two new mutations in phage X that produce clear plaques instead ofturbid plaques of wild-type X phage. The two mutants are infected together into an E. colhost at a multiplicity of infection that ensures that each E. coil ceil receives at least one phageof each type. The phage produced from this mixed infection are plated and of 1000 plaquesexamined, 997 are clear and 3 are turbid.(a 10 pts.) What is the distance between the two clear plaque mutations in map units?_" " _ L_ _C#__.5 _ - :7- - j.. /,,. c._,¢_.ISt-Ct'C_L= _ rca.om:bi _,_oc_SX loxD y.. b _%,LL ' 'TbT_ lOGO ........ x._ ._ -- hcf"f" • " • )-i"tq¢3t-uuf_4?_4x.s 0a'_ _Eo_rtba_%uE Ccu@-?srza_chChub'badf_)L__oI?_.L_,I_S_/bF£._&_-_CLOLkfL_ _L_3..L. A..LCEY4t_ j,.'_f_%{...-[lv%LS,_1.2;T¢---/,_.<_u_t-4j YEo-.m_Y_ct4,t,_s.(b 5 pts.) If the two clear plaque mutations were found by a complementation test to be in twodifferent closely linked genes, would this change the calculated distance between the twomutations? Explain why or why not.NO. t_'LL 6L,{S-'t-EY_ce_"_AC£,iR.Silf_£ £_¢¢_..t _0,LC/_3.L,.LbO'th,,t CSL,L4LLLE-L--t(c 10 pts.) In


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