Exams Fall 1998 O t t b 7 03 Exam 1 lCL d Name Section Exam TA starts at 11 05 and ends at 11 55 There are six pages including this cover page Please write your name on each page Please Look over the entire exam so you don t spend too much time on hard questions leaving easy questions unanswered Check your answers to make sure that they make sense To help us give partial credit show your work and any assumptions that you make Question Question Question 1 2 3 30 points 35 points 35 points Name 1 The genes for two human autosomal dominant traits are 10 cM apart as determined by meiosis Q in females individual In the following pedigrees with trait 1 Q individual the traits are indicated with trait 2 G as follows individual with trait 1 and trait 2 a 15 pts For each of the pedigrees shown below calculate the probability that the individual designated by will have either dominant trait 1 dominant trait 2 or both traits t 4 4 21 r Probability Dominant trait 1 only I 4 Dominant trait 2 only i 4 Both trait 1 and trait 2 1 b 15 pts 2 Probability Dominant trait 1 only Dominant trait 2 only c1 4 4 Both trait 1 and trait 2 i tZ O Name 2 Wild type Drosophila have red eyes and white eyes are the result of an X linked recessive mutation A new recessive mutation that gives apricot colored eyes is isolated A female from a true breeding apricot strain is crossed to a mate from a true breeding white strain and all of the F1 females have pale apricot eyes and all of the males have apricot eyes a 5 pts Are the white and apricot mutations Explain your answer la in the same gene or in different genes dj nc qT vD q n s do o compidm r t f roqnv f qStj Gam tcnl nt fhOG qa rddl A collection of F1 females from the cross described above all with pale apricot eyes are crossed to males from a true breeding white eyed strain and 1000 progeny are examined Among these progeny 6 flies have normal red eyes b 5 pts What is the measured Sadra r Z rd omt tnt T0t l racomt4n r t b t n distance between the white and apricot mutations C A S rc JcclCS in cM r Jottb d ttC nt LL Ct l 2 0 x too iooo l zclVt A new recessive eye color mutation known as peach is isolated A female from a truebreeding peach strain is crossed to a male from a true breeding white strain and all of the F1 females have normal red eyes and all of the males have peach eyes c 5 pts Is the peach mutation on an autosome answer or on the X chromosome Explain your a mcd s d 5 pts Are the white and peach mutations in the same gene or in different genes Explain your answer t reA eqed n q le s Name The recessive mutation for crossveiniess wings lies on the X chromosome A female from a true breeding strain with apricot eyes and crossveintess wings is crossed to a male from a true breeding strain with white eyes and normal wings As expected all of the F1 females from this cross have pale apricot eyes and norrnal wings A large collection of these F1 females are crossed to wild type males and 10 000 male progeny are examined The observed phenotypes are as follows Phenotype Number normal wings cv wings white eyes apricot eyes normal wings cv wings apricot eyes white eyes normal wings red eyes cv wings red eyes 4 418qI 4 330 610 4 iOz d O 3SS dOf 590J weoR W tq C v 2 u4ObLbJoC b O JC 50 ilq C O SeVdfbd dd d tq e 15 pts Draw a genetic map showing the relative order and distances in cM between the crossveinless apricot and white mutations i o z 04 z 4 q 4 9 w CV a p w c tJ t aec 2 tz 2 ts o oq oo i o4 cN 0 t iOl bOO c oo 11 o4io oOo clV Name 3 a5 pts In a yeast cross the segregation of two mutations are followed Amo j4he 3 i A b 5 pts In another cross involving two different mutations PD 40 T 8 NPD 2 Give as much information as you Can about the chromosomal location of these two mutations 20c You have isolated two new yeast mutations big1 is a mutation that produces colonies that are larger than normal and smll is a mutation that produces colonies that are smaller than normal e wild type colony big1 colony smtl colony A big1 mutant is mated to a smll mutant of the opposite mating type the resulting diploid is sporulated and 12 tetrads are dissected The tetrad dissection plate looks like this o o o o e o o o o o o 1 e o o Name c 5 pts What is the phenotypeof a big1 stall doublemutant Does it look like wild type a big1 mutant or a stall mutant 4 d 10 pts 4 Among the t2 tetrads shown above how many tetrads are there of each type PD T 7 NPD 2 What is the linkagerelationshipbetweenbig1 and stall Give distancesin cM if applicable L Ur V dd b 7 03 E am 2 Name Section Exam TA starts at 11 05 and ends at 11 55 There are seven pages including this cover page Please write your name on each page Please o Look over the entire exam so you don t spend too much time on hard questions leaving easy questions unanswered o Check your answers to make sure that they make sense To help us give partial credit show your work and any assumptions that you make Question Question Question 1 2 3 35 points 30 points 35 points i l 1 J l 1 11h e turbid You have isolated two newX mutations in two phagemutants X that are produce clear plaquesinto instead of plaques of wild type phage The infected together an E col host at a multiplicity of infection that ensures that each E coil ceil receives at least one phage of each type The phage produced from this mixed infection are plated and of 1000 plaques examined 997 are clear and 3 are turbid a 10 pts What is the distance between the two clear plaque mutations in map units C L ISt Ct rca om bi oc SX loxD TbT L y C 5 b LL lOGO 7 x j c hcf i tq 3 t uuf 4 4x s 0a Eo rtba uE Ccu srza chChub bad f f L oI L I S bF CLOLkfL L 3 L A LCEY4t j f lv LS 1 2 T u t 4j YE o m Y ct4 t s b 5 pts If the two clear plaque mutations were found by a complementation test to …
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