Pyle April 22 Regulation of Prokaryotic Transcription 547-560LIFE SCIENCES 3 (LS 3.2) Introduction to Molecular Biology, Spring 2010 Tue & Thu, 2-3;15pm, La Kretz 110 Dr. April Pyle & Dr. Randolph Wall Lecture Date Topics Reading Pyle March 30 Molecular Components 43-56 Pyle April 1 Protein structure 71-94 Pyle April 6 Protein function & Techniques 764-774 Pyle April 8 DNA Structure 101-126 Pyle April 13 DNA Replication 195-255 Pyle April 15 RNA structure 127-133 Pyle April 20 Transcription 377-414 Pyle April 22 Regulation of Prokaryotic Transcription 547-560 Pyle April 27 Regulation of Eukaryotic Transcription 589-631 Pyle April 29 Midterm Exam Review Wall May 4 Eukaryotic Genes & mRNA Processing 406-410, 415-425, 430-435, 452-453 Pyle May 5 Midterm Exam (5-7:50pm) Wall May 6 Translation I: Genetic Code & tRNA 457-469, 521-538 Wall May 11 Translation II: Ribosomes & Protein 469-496, 502-503 Synthesis Wall May 13 Viruses & the New Central Dogma 783-794 Wall May 18 Restriction Enzymes & DNA Mapping 739-746 Wall May 20 Recombinant DNA Cloning & PCR 746-752 Wall May 25 Screening Libraries & Microarrays 743-749, 662 Wall May 27 Sequencing Genes & Genomes/The 641-655, 659, Transcriptome & RNA Silencing 703-707, 753-764 Wall June 1 Comparative Genomics 711-715 Wall June 3 Final Exam Review Wall June 9 Final Exam (11:30-2:30pm) Text: Molecular Biology of the Gene (Sixth Edition), Cold Spring Harbor Press. J. D. Watson, T.A. Baker, S. P. Bell, A. Gann, M. Levine, & R. Losick Note: LS3 is an impacted course. Students have up to Friday of Week 2 to drop this course.Instructors: Dr. April Pyle Dr. Randolph Wall Office Hours: Dr. Pyle (Tuesdays 3:30-5PM @ BSRB 277A) Dr. Wall (Tuesdays 3:30-5PM @ Boyer 529) TAs: Feng, Jiaying ([email protected]) Huang, Tsung-Pai (James) ([email protected]) Ulgherait, Matthew ([email protected]) Yang, Gang (Alvin) ([email protected]) Yun, Jina ([email protected]) Zhou, Xi (Josie) ([email protected]) Websites: http://www.lsic.ucla.edu/classes/spring10 http://lslab.lscore.ucla.edu Lab Management: Dr. Gaston Pfluegl. Slichter 2875 ([email protected]) LS Core Student Affairs Officers: Lily Yanez. LS 2305 ([email protected]) Jane Park. LS 2305 ([email protected]) Grading: Midterm Exam on 05/05 (5-6:50PM) 200 points Labs 100 points Final Exam on 06/09 (11:30-2:30PM) 200 points (20 points of the final exam will pertain to lab material) Total: 500 points Make-up Policy: No make up examination will be given. If you are unable to take an examination due to illness or other emergency, you are responsible for contacting the Life Sciences Core Curriculum Office located in LSB 2305 before the examination. You are required to have written verification from a physician regarding the illness or