LS3-2 SYLLABUS* Fall 2006 (Silver) Handout Date Major Concepts Readings (MBoC) ** Unity and diversity of cells, Cell shave similar basic chemistry pp. 3-12, 16-18, 29, 58-59 Discovery of cells & importance of the microscope pp. 547-563 Model organisms pp. 27-28, 35-40, 42-44 Chemical foundations: atoms, isotopes, molecules, bonds, water pp. 47-58, 112-115, 56-57 (review) Phospholipid bilayer pp. 584-586, 118-119, Fig 2-22 Amino acids are the building blocks of proteins pp. 62-63, 132-133 Proteins: Sequence, structure, function pp. 129-141, 147-8, 151-2, 593-6 (skim) Protein folding , misfolding, and degradation pp. 134-135, 355-360, 362-363 Sequence alignments, BLAST, homology pp. 144-5, 159, 517-8, Fig 1-52 Interaction of proteins with other molecules (binding sites, affinity, specificity) pp. 156-162 Antibodies pp. 160, 1375 – 6 (skim), 1381-1384 Enzymes, Biochemical energetics, ΔG pp. 75-76, 68-71, 79, 162 Regulation of protein function pp. 171-178 Techniques: Protein isolation and analysis pp. 513-515, 481-491 + handout DNA is the genetic material pp. 191-192 + handout Structure and function of DNA pp. 63-65, 192-197, 120-121 Structure of eukaryotic chromosomes, packing of DNA pp. 198-201, 207-12, Fig 4-55 Chromatin remodeling pp. 212-216 DNA replication pp. 238-241, 244-249, 253-258, 263-6 Activated carrier molecules, coupled reactions pp. 81-85Intro to DNA techniques, Restriction enzymes, Gel electrophoresis pp. 491-495 Recombinant DNA techniques, cloning, PCR, DNA sequencing pp. 500-510 + Fig 8-41 …. Introduction to RNA pp. 63-65, 304-305 Basic transcription pp. 299-312 Genome organization pp. 200-204 Regulation of gene expression at the transcriptional level pp. 375-384, 312-313, 395-413 Post-transcriptional regulation of gene expression pp. 363-364, skim 448-449 & 358-362 RNA processing pp. 315-320, 326-329, 436-438 (skim) Translation pp. 335-352, 354-355 • *The lecture handouts will contain the most up-to-date information about assigned readings – NOT the syllabus • **Unless otherwise noted, all Readings are from Molecular Biology of the Cell (MBoC), 4th Edition by Alberts, et.