Slide 1Slide 2Slide 3Slide 4Slide 5Slide 6Slide 7Slide 8Slide 9Slide 10Slide 11Slide 12Slide 13Slide 14Slide 15Slide 16Slide 17Slide 18Slide 19Slide 20Fusion Proteins and Mechanisms of ActionSynaptic vesicle fusion: SNARES and C2 domainsViral Fusion: Class I and Class II Fusion ProtiensInfluenza hemagglutininHA is class I viral fusion protein.HIV, SIV, MoMLV, HTLV, SV5, HRSV and Ebola are also class 1. All have trimeric bundle of helical hairpin with the N and C termini pointing in the same direction (fusion active form – low pH).Note: X-ray of most fusion proteins are non-complete forms, that is the transmembrane segments are removed for aid in crystallization6 helical bundle: target of many inhibitory drugsClass II viral membrane fusion proteins consist of three domains and form dimers on the membrane surface. pH in the endosome is the fusion “trigger”.The dimer changes to trimer.These proteins are mostly beta sheet in secondary structure.The “fusion” peptide is a short loop that protrudes from the tips of the dimerization domainSemliki virus fusion
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