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UF CHM 6304 - Lecture notes

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Slide 1Slide 2Slide 3Slide 4Slide 5Slide 6Slide 7Slide 8Slide 9Slide 10Slide 11Slide 12Slide 13Slide 14Slide 15Slide 16Slide 17Slide 18Slide 19Slide 20Slide 21Analytical UltracentrifugationDensity Gradient formed from various solutes:RAFT MODEL‘70s‘40s ‘50sCurrent dayThe hydrophobic effect:Transfer from water to alcoholMLVsSUVsMultilamellar vesiclesunilamellar vesicles50nm 500nm 5000nmAs vesicles get smaller, they tumble more quickly, have a higher surface curvature, less interior volume, are less thermodynamically stable, and less symmetry in lipid distribution Liposomes as Membrane MimeticsFreeze-fracture electron microscopyBlack Lipid BilayersOther membrane mimetics:MLVs, SUVs, LUVs (vesicles)MicellesBicellesBlack Lipid BilayersLangmuir MonolayersSupported BilayersMLVsSUVsunilamellar vesiclesMicelleDetergents,Shortchain lipidslysolipidsLangmuir MonolayersSupported Lipid BilayersBicelles4:1 DMPC:DHPC1:2 DMPC:DHPCFigures taken from: Langmuir 2003, 19, 6936Phase dependent upon relative concentrations of long chain and short chain lipids and


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UF CHM 6304 - Lecture notes

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