Analytical UltracentrifugationDensity Gradient formed from various solutes:RAFT MODEL‘70s‘40s ‘50sCurrent dayThe hydrophobic effect:Transfer fromwater to alcoholMLVsMultilamellar vesiclesSUVsunilamellar vesicles50nm 500nm 5000nmAs vesicles get smaller, they tumble more quickly, have a higher surface curvature, less interior volume, are less thermodynamically stable, and less symmetry in lipid distribution Liposomes as Membrane MimeticsFreeze-fracture electron microscopyBlack Lipid BilayersOther membrane mimetics:MLVs, SUVs, LUVs (vesicles)MicellesBicellesBlack Lipid BilayersLangmuir MonolayersSupported BilayersMLVsSUVsunilamellar vesiclesMicelleDetergents,Shortchain lipidslysolipidsLangmuir MonolayersSupported Lipid BilayersBicelles4:1DMPC:DHPC1:2DMPC:DHPCFigures taken from: Langmuir 2003, 19, 6936Phase dependent upon relative concentrations of long chain and short chain lipids and
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