Slide 1Slide 2Slide 3Slide 4Slide 5Slide 6Slide 7Slide 8Slide 9Slide 10Slide 11PE: Hydration, enthalpy and entropyIntermediate structures Between Phases31P Solid State NMR of lipid assembliesBreath, anisotropy and isotropic chemical shiftsSolution Vs Solid State NMRFast isotropic tumbling Slow to no molecular reorientation•Isotropic chemical shifts•narrow lines•J couplings•Chemical shift powder patterns•broad lines•dipolar broadening•quadrupolar broadening2H NMR - quadrupole nuclei31P NMR- S=1/2 nucleiMagnetic Anisotropic InteractionsChemical shielding/chemical shiftChemical Shift Dependence Upon OrientationIntegrate over all space for a “powder”MLVs and GUVs act as powders. They are large enough to tumble slowly enough NOT to average the CSA and 2H quad interactionChemical Shift Dependence for CrystalsDry powder•Restricted motions•mixtures of different lipids•mixtures of different phases•exchange ratesExchange Between Two Sites: Effects on NMR LineshapesTwo states: A and BHydration effects (entropic) on L to HII phase for
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