Slide 1Slide 2Slide 3Slide 4Slide 5Lab 19 Goals and Objectives:Exercise 21: EnumerationCount colonies (30-300 plates) and share data(Sample data/explanations in supplemental packet pages 69-72)Exercise 58: Bacterial Counts on FoodsPlate pre-made dilutions for the following foods: meat, spinach, and milkUse pre-poured solid plates and spread with beadsEach pair needs: 1:10 of each: meat, spinach, milk (no blending: done!)1:1000 of each: meat, spinach, milk (no diluting: done!)9 NA plates and 9 tubes of beadsExercise 59: Bacteriological Examination of WaterIf turbid, pre-filter water with cheese-cloth in funnel into new bottleEach pair set up tubes for one water sample (you need minimum 200ml in your sample for this exercise and Ex. 60)Each pair needs: 5 DS lactose broth LARGE tubes 10 SS lactose broth normal tubesExercise 60: The Membrane Filter MethodEach pair use the same water sample from Exercise 59Perform according to directions pg 403 except skip step 4, Filter 100ml of sampleEach pair needs: Filter apparatus, Grid filter disc, Absorbent pad, Petri dish, Endo MF broth0.10.20.30.4y = mx+b3.4 X 1062.55 X 105CFU/mlOnce you can assign real CFU/ml numbers to the OD readings, you have a standard curve and the equation of the line can be used to determine the CFU/ml concentration in a sample from the OD readingFig 58.1Dilutions already made, you just plateFigure 59.1Lab 19 Goals and Objectives:Exercise 21: EnumerationCount colonies (30-300 plates) and share data(Sample data/explanations in supplemental packet pages 69-72)Exercise 58: Bacterial Counts on FoodsPlate pre-made dilutions for the following foods: meat, spinach, and milkUse pre-poured solid plates and spread with beadsEach pair needs: 1:10 of each: meat, spinach, milk (no blending: done!)1:1000 of each: meat, spinach, milk (no diluting: done!)9 NA plates and 9 tubes of beadsExercise 59: Bacteriological Examination of WaterIf turbid, pre-filter water with cheese-cloth in funnel into new bottleEach pair set up tubes for one water sample (you need minimum 200ml in your sample for this exercise and Ex. 60)Each pair needs: 5 DS lactose broth LARGE tubes 10 SS lactose broth normal tubesExercise 60: The Membrane Filter MethodEach pair use the same water sample from Exercise 59Perform according to directions pg 403 except skip step 4, Filter 100ml of sampleEach pair needs: Filter apparatus, Grid filter disc, Absorbent pad, Petri dish, Endo MF
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