Slide 1Slide 2Slide 3Slide 4Slide 5Slide 6Lab 27 Goals and Objectives:**NO DISINFECTING, NO HAND WASHING before 33&36!!!**Exercise 31: Lethal Effect of UV LightScore plates (+++, ++, +, -) for growth using control side of each plate as +++ for comparison; record on class sheetExercise 33: Effectiveness of Alcohol as an AntisepticEach person do, follow supplemental packet directions p.113Exercise 36: Effectiveness of Hand ScrubbingFour groups total: each group elect a scrubber and scrubber assistant, Others do plating; Use solid pre-poured plates, plate using flame sterilized spreader, 10 plates per group:1 tap water control (0.4ml), 3 basin A (0.1ml, 0.2ml, 0.4ml)3 basin B (0.1ml, 0.2ml, 0.4ml), 3 basin C (0.1ml, 0.2ml, 0.4ml)Exercise 34: Antimicrobial Sensitivity TestingFour groups: each test all 8 antibiotics against two organisms (assigned), two plates per organism, 4 discs per plate. Exercise 35: Evaluation of AntisepticsEach try five agents against both organisms S. aureus & P. aeruginosaSAME five agents for both to compare Gram pos. vs. neg.Effectiveness of Alcohol as an AntisepticDivide plate into 6 wedgesA BCDEFA = left/non-dominant thumb dirtyB = left/non-dominant thumb again (how many microbes removed just by touching agar?)C = right/dominant thumb dirtyD = right/dominant thumb dipped in alcohol and allowed to dry (how many microbes removed/killed by alcohol dip?E = right/dominant index fingerF = right/dominant index finger wiped with alcohol swab (how many microbes removed/killed by swabbing with alcohol?)Replacement Directions for Exercise 33Supp. page 125Fig. 36.1Stop at step 5 while you still have skin!10 plates total:1 tap water control (0.4ml), 3 basin A (0.1ml, 0.2ml, 0.4ml)3 basin B (0.1ml, 0.2ml, 0.4ml)3 basin C (0.1ml, 0.2ml, 0.4ml)Exercise 34: 2 plates each organism, 4 discs per plate Each organism gets tested against 8 different antibiotics Group 1 Staphylococcus aureus & Escherichia coli Group 2 Staphylococcus aureus & Proteus vulgaris Group 3 Proteus vulgaris & Pseudomonas aeruginosa Group 4 Escherichia coli & Pseudomonas aeruginosaLab 27 Goals and Objectives:**NO DISINFECTING, NO HAND WASHING before 33&36!!!**Exercise 31: Lethal Effect of UV LightScore plates (+++, ++, +, -) for growth using control side of each plate as +++ for comparison; record on class sheetExercise 33: Effectiveness of Alcohol as an AntisepticEach person do, follow supplemental packet directions p.113Exercise 36: Effectiveness of Hand ScrubbingFour groups total: each group elect a scrubber and scrubber assistant, Others do plating; Use solid pre-poured plates, plate using flame sterilized spreader, 10 plates per group:1 tap water control (0.4ml), 3 basin A (0.1ml, 0.2ml, 0.4ml)3 basin B (0.1ml, 0.2ml, 0.4ml), 3 basin C (0.1ml, 0.2ml, 0.4ml)Exercise 34: Antimicrobial Sensitivity TestingFour groups: each test all 8 antibiotics against two organisms (assigned), two plates per organism, 4 discs per plate. Exercise 35: Evaluation of AntisepticsEach try five agents against both organisms S. aureus & P. aeruginosaSAME five agents for both to compare Gram pos. vs.
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