BME 400 BME Capstone Design Date 10 8 06 Title Microencapsulation of tissues and cells for treatment of hormone related diseases microencapsulation Project 4 Client Dr Craig Atwood GRECC 11G VA Hospital Faculty Advisor Professor William Murphy 2154 ECB Team Members Albert Kwansa Team Leader John Harrison Team Communicator Yik Ning Wong Jacqueline BSAC Representative Eric Lee BWIG Dates 10 2 06 10 8 06 Week 4 Project Design Statement To design polyethylene glycol diacrylate PEGDA hydrogels for the encapsulation of Murine Leydig Tumor Cells in an effort to increase cell viability and testosterone secretion The PEGDA hydrogels must provide immunoprotection and allow for the effective diffusion of oxygen nutrients hormones and metabolic wastes The overall goal is to restore the testosterone level of castrated mice via injection or implantation of the PEGDA encapsulated cells Restatement of Previous Team Goals 1 Present individual research to the rest of the team at 10 2 team meeting 2 Continue to develop problem design statement PDS 3 Begin brainstorming design ideas Current Individual Goals Albert Kwansa Team Leader Work on 4th progress report due Sunday 10 8 06 by 5pm Begin collecting and drafting lab protocols for experiments John Harrison Team Communicactor Work with Eric to obtain information about pricing delivery and storage of reagents materials and kits needed for the proposed experiments Yik Ning Wong Jacqueline BSAC Representative Continue to research components that make up the extracellular matrix ECM of leydig cells for possible incorporation in the hydrogel to promote cell adhesion and secretion of hormones i e adhesion molecules Also look into UV exposure time UV wavelength and their effects on cell viability Eric Lee BWIG Work with John to obtain information about pricing delivery and storage of reagents materials and kits needed for the proposed experiments 1 BME 400 BME Capstone Design Date 10 8 06 Summary of Team Accomplishments 1 Established three possible areas to focus on namely PEGDA hydrogel thickness and its influence on effective diffusion adhesion peptides to promote cell viability and or testosterone production and UV photopolymerization time and intensity and its effects on cell viability Summary of Current Team Goals 1 Present individual research to the rest of the team at 10 8 team meeting 2 Development of a proposal to submit to our client including specific goals for the semester proposed experiments and funding requests Questions and Concerns The project builder for BWIGs is currently not functioning properly Important Notes 1 The mid semester presentations have been changed from Fri 10 20 06 to Fri 10 27 06 for BME 400 only 2 Design notebooks are now due Wed 11 1 06 Fall Semester Schedule Week 1 2 3 4 Goals From BME Design Schedule Day Date Activities M T W R 9 4 9 5 9 6 9 7 F 9 8 Take team photo General research of project topic Review past reports and presentations M 9 11 Team meeting 1 on Mon 9 11 at 11 am in ECB Develop list of questions for first client meeting T W R F M T W R F 9 12 9 13 9 14 9 15 9 18 9 19 9 20 9 21 9 22 M 9 25 T W R 9 26 9 27 9 28 F 9 29 Labor Day Form teams Select project Contact client Email team roles to advisor Literature search Meet w Client Client meeting 1 on Fri 9 15 at 12 00 pm in GRECC VA Hospital Advisor meeting 1 on Mon 9 18 at 4 pm near 2154 ECB Team meeting 2 on Thurs 9 21 at 7 00 pm in ECB Develop understanding of project Develop PDS Team meeting 3 on Mon 9 25 at 2 30 pm in ECB Advisor meeting 2 on Mon 9 25 at 3 30 pm near 2154 ECB Team meeting 4 on Tues 9 26 at 8 15 pm in ECB Brainstorm Advisor meeting 3 on Fri 9 29 at 1 00 pm near 2154 ECB Evaluate current direction 2 BME 400 BME Capstone Design Week 5 6 7 8 9 10 11 12 13 Day Date M 10 2 T W R F M T W R F M T W R F M T W R 10 3 10 4 10 5 10 6 10 9 10 10 10 11 10 12 10 13 10 16 10 17 10 18 10 19 10 20 10 23 10 24 10 25 10 26 F 10 27 M T W R F M T W R F M T W R F M T W R F M T W R F 10 30 10 31 11 1 11 2 11 3 11 6 11 7 11 8 11 9 11 10 11 13 11 14 11 15 11 16 11 17 11 20 11 21 11 22 11 23 11 24 11 27 11 28 11 29 11 30 12 1 Date 10 8 06 Activities Team meeting 5 on Mon 10 2 at 2 30 pm in ECB Advisor meeting 4 on Mon 10 2 at 3 30 pm near 2154 ECB Goals Design Alternatives Team meeting 6 on Sun 10 8 at 7 00 pm in ECB Advisor meeting 5 on Mon 10 9 at 3 30 pm near 2154 ECB Work on Mid Semester Presentation Report Lecture Oral Presentation Guidelines Peer Review of Report Drafts Work on Mid Semester Presentation Decide on Final Design MID SEMESTER PRESENTATIONS NEW BME 400 Only Lecture Overview of Shop Facilities Design Notebooks Due to Advisor NEW Work on Design Work on Design Lecture Intellectual Property Lecture Work on Design Work on Design THANKSGIVING Work on Design 3 BME 400 BME Capstone Design Week 14 15 Day M T W R F M T W R F Date 12 4 12 5 12 6 12 7 12 8 12 11 12 12 12 13 12 14 12 15 Date 10 8 06 Activities Goals Work on Presentation FINAL POSTER PRESENTATIONS Final Report Design Notebooks Due to Advisor Final Meeting w Advisor 4