Problem StatementWhat is FNA?Slide 3Product Design SpecificationsCurrent PrototypeTissues TestedTesting ProceduresSample QuantificationFuture WorkDrill Bit Size ComparisonTissue Sample- MammaryTissue Sample- LymphTissue Sample- FatOld Technique- MammaryOld Technique- LymphOld Technique- FatNew Technique- MammaryNew Technique- LymphNew Technique- FatProblem StatementProblem StatementGOAL:GOAL: To modify the needle used during a To modify the needle used during a Fine Needle Aspiration (FNA) procedure. Fine Needle Aspiration (FNA) procedure. The modification should not drastically The modification should not drastically change the FNA procedure and still change the FNA procedure and still increase the amount of tissue removed for increase the amount of tissue removed for cytological testing. It should also minimize cytological testing. It should also minimize the time (by collecting an adequate sample the time (by collecting an adequate sample size on the first attempt) and discomfort size on the first attempt) and discomfort caused to the patient during the procedurecaused to the patient during the procedure. .What is FNA?What is FNA?Technique used to Technique used to extract cells; no extract cells; no incision neededincision neededSeveral insertions are Several insertions are usually required to usually required to ensure an adequate ensure an adequate sample sizesample sizeSamples are sent to Samples are sent to pathologist to be pathologist to be analyzedanalyzedNational Breast Cancer Center, http://www.NBCC.orgFine Needle Aspiration (FNA)A.D.A.M., http://www.adam.comProduct Design SpecificationsProduct Design SpecificationsRemoves adequate number of cells for Removes adequate number of cells for diagnosis.diagnosis. Should not cause more discomfort than Should not cause more discomfort than current FNA procedure.current FNA procedure. Constrained to standard 20-25-gauge Constrained to standard 20-25-gauge needle, client prefers 23-gauge.needle, client prefers 23-gauge.Current PrototypeCurrent PrototypePre-manufactured drill bitPre-manufactured drill bit6” length6” lengthFits in 23 gauge needleFits in 23 gauge needleTissues TestedTissues TestedFixed cat tissuesFixed cat tissues–MammaryMammary–LymphLymph–FatFatTesting ProceduresTesting ProceduresOld techniqueOld technique–No insert usedNo insert used–Performed two separate trials on all three Performed two separate trials on all three tissuestissuesNew techniqueNew technique–Drill bit insert usedDrill bit insert used–Performed two separate trials on all three Performed two separate trials on all three tissuestissuesObserved in saline bath under microscopeObserved in saline bath under microscopeSample QuantificationSample QuantificationOld techniqueOld technique–Resulted in either a mass of cells or a Resulted in either a mass of cells or a minimal quantity of cells minimal quantity of cells New techniqueNew technique–More dispersed cell sampleMore dispersed cell sample–Greater number of cells on averageGreater number of cells on averageFuture WorkFuture WorkFurther prototype developmentFurther prototype developmentResearch into appropriate materials to be Research into appropriate materials to be used (stainless steel, Co-Cr, Ti)used (stainless steel, Co-Cr, Ti)Develop protocol for quantifying testingDevelop protocol for quantifying testingTest on freshly removed tissueTest on freshly removed tissueIn vivoIn vivo testing of animal tumors testing of animal tumorsCytological evaluationCytological evaluationDrill Bit Size ComparisonDrill Bit Size ComparisonTissue Sample- MammaryTissue Sample- MammaryTissue Sample- LymphTissue Sample- LymphTissue Sample- FatTissue Sample- FatOld Technique- MammaryOld Technique- MammaryOld Technique- LymphOld Technique- LymphOld Technique- FatOld Technique- FatNew Technique- MammaryNew Technique- MammaryNew Technique- LymphNew Technique- LymphNew Technique- FatNew Technique-