Title Microencapsulation of Tissues and Cells Team Members Joe Zechlinski Team Leader Bryan Baxter Communications Timothy Eng BWIG Representative April Zehm BSAC Representative Client Craig Atwood Ph D Research Director UW Memory Research Program University of Wisconsin Madison Medical School Research Director Wisconsin Alzheimer s Institute William S Middleton Memorial Veterans Hospital GRECC 11G Phone 608 256 1901 ext 11664 Fax 608 280 7291 E mail csa medicine wisc edu Date 11 04 05 11 10 05 Problem Statement A method of treatment for various diseases incorporates the encapsulation of cells and tissues and the time released delivery of chemical mediators Presently this method encounters a slew of problems including a lack of biocompatibility limited immunoprotective properties and hypoxia The client desires the development of microcapsules that would permit the successful release of hormones namely testosterone and inhibin by encapsulated cells into animals while avoiding the aforementioned problems Website http homepages cae wisc edu bme200 microencapsulation fall05 Last Week s Goals Crosslink PEG microcapsules with and without cells This Week s Goals Construct 2 new microfluidic devices Keep attempting UV crosslinking Think of better ways to isolate capsules after crosslinking Summary of Accomplishments Attempted crosslinking using stationary UV in Masters lab Capsules exposed to approx 15 sec UV light 4mW cm2 intensity in vicinity of microfluidic device Difficult to assess whether air bubbles or PEG spheres were being viewed under microscope Met with R Haasl M Gallego and S Vadakkadath Meethal at VA client meeting Discussed working in two groups after successful UV crosslinking with microfluidics was accomplished one focusing on biology cell material aspects of project and other focused on improving engineering fluidics aspects Difficulties Difficult to isolate and find microspheres after they come out of microfluidic device and deposit into beaker or Petri dish Difficult to determine whether they are sufficiently crosslinked Activities Team 5 25 hr team advisor meeting 15 min client meeting 1 hr UV testing with microfluidic device making new devices 4 hr Joe Zechlinski 5 25 hr progress report 15 min cell culture 4 hr design notebook 1 hr Bryan Baxter 1 hr design notebook Tim Eng 1 hr design notebook April Zehm 2 hr design notebook 1 hr cell culture 1 hr Total time this week 14 5 hours Cumulative Project time 99 0 hours
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