BME 400 BME Capstone Design Date 12 10 06 Title Microencapsulation of tissues and cells for treatment of hormone related diseases microencapsulation Project 4 Client Dr Craig Atwood GRECC 11G VA Hospital Faculty Advisor Professor William Murphy 2154 ECB Albert Kwansa Team Leader Team Members John Harrison Team Communicator Yik Ning Wong Jacqueline Biomedical Student Advisory Committee BSAC Representative Eric Lee Biomedical Web Implementation Group BWIG Representative Project Webpage http homepages cae wisc edu bme200 microencapsulation f06 Dates 12 4 06 12 10 06 Weeks 14 Progress Report 12 Project Design Statement To design polyethylene glycol diacrylate PEG dA hydrogels for the encapsulation of Murine Leydig Tumor Cells in an effort to increase cell viability and testosterone secretion The PEGDA hydrogels must provide immunoprotection and allow for the effective diffusion of oxygen nutrients hormones and metabolic wastes The overall goal is to restore the testosterone level of castrated mice via injection or implantation of the PEG dA encapsulated cells Restatement of Previous Team Goals 1 Perform Cell Titer Blue Cell Viability Assay on cells to be obtained on 12 4 06 and evaluate the effects of exposure time 365 nm UV light 2 Complete lyophilization of PEGdA samples and perform photopolymerization initially to observe whether they are any further complications with gelation 3 Continue research on experimental parameter rationalization and use this research in conjunction with UV cell experimental results to propose an optimized design for the process of developing PEGdA 12000 gels for encapsulation 4 Continue to develop presentation for final poster session and writing of the final report Summary of Team Accomplishments 1 Performed Cell Titer Blue Cell Viability experiments and PEGdA hydrogel swelling studies 2 Presented during final poster session on Fri 12 8 06 at 12 16pm in ECB atrium Current Individual Goals Albert Kwansa Team Leader Work on 12th progress report due Sunday 12 10 06 by 5pm John Harrison Team Communicator Yik Ning Wong Jacqueline BSAC Representative Eric Lee BWIG Representative Summary of Current Team Goals 1 Complete the final report and prepare design notebooks for the 2nd evaluation due Wednesday 12 13 06 2 Gather lab supplies determine a location for storage and keep an inventory of all materials reagents and assay kits and their locations for next semester 1 BME 400 BME Capstone Design Fall Semester Schedule Week 1 2 3 4 5 6 7 8 Day M T W R Date 9 4 9 5 9 6 9 7 F 9 8 M 9 11 T W R F M T W R F 9 12 9 13 9 14 9 15 9 18 9 19 9 20 9 21 9 22 M 9 25 T W R 9 26 9 27 9 28 F 9 29 M 10 2 T W R F M T W R F 10 3 10 4 10 5 10 6 10 9 10 10 10 11 10 12 10 13 M 10 16 T W R F 10 17 10 18 10 19 10 20 M 10 23 T W 10 24 10 25 R 10 26 F 10 27 Date 12 10 06 Activities Goals From BME Schedule Labor Day Take team photo General research of project topic Review past reports and presentations Team meeting 1 on Mon 9 11 at 11 am in ECB Develop list of questions for first client meeting Form teams Select project Contact client E mail team roles to advisor Literature search Meet w Client Client meeting 1 on Fri 9 15 at 12 00 pm in GRECC VA Hospital Advisor meeting 1 on Mon 9 18 at 4 pm near 2154 ECB Team meeting 2 on Thurs 9 21 at 7 00 pm in ECB Develop understanding of project Develop PDS Team meeting 3 on Mon 9 25 at 2 30 pm in ECB Advisor meeting 2 on Mon 9 25 at 3 30 pm near 2154 ECB Team meeting 4 on Tues 9 26 at 8 15 pm in ECB Brainstorm Advisor meeting 3 on Fri 9 29 at 1 00 pm near 2154 ECB Evaluate current direction Team meeting 5 on Mon 10 2 at 2 30 pm in ECB Advisor meeting 4 on Mon 10 2 at 3 30 pm near 2154 ECB Design Alternatives Team meeting 6 on Sun 10 8 at 7 00 pm in ECB Advisor meeting 5 on Mon 10 9 at 3 30 pm near 2154 ECB Work on Mid Semester Presentation Report Lecture Oral Presentation Guidelines Peer Review of Report Drafts Team meeting 7 on Mon 10 16 at 2 30 pm in ECB Advisor meeting 6 on Mon 10 16 at 3 30 pm near 2154 ECB Work on Mid Semester Presentation Team meeting 8 on Fri 10 20 at 12 00 pm in ECB Team meeting 9 on Mon 10 23 at 3 20 pm in ECB Advisor meeting 7 on Mon 10 23 at 3 30 pm near 2154 ECB Team presentation development in CAE at 5 30 pm Team presentation development in ECB at 5 45 pm Team presentation practice at 9 30 pm in room 1047 ECB reserved through Student Leadership Center MID SEMESTER PRESENTATIONS NEW BME 400 Only Design Notebooks Due to Advisor NEW Decide on Final Design 2 BME 400 BME Capstone Design 9 10 11 M 10 30 T W R F 10 31 11 1 11 2 11 3 M 11 6 T W R 11 7 11 8 11 9 F 11 10 M 11 13 T W R 11 14 11 15 11 16 F 11 17 M T W R F M T 11 20 11 21 11 22 11 23 11 24 11 27 11 28 W 11 29 R F 11 30 12 1 S 12 2 N M T 12 3 12 4 12 5 W 12 6 R F M T W R F 12 7 12 8 12 11 12 12 12 13 12 14 12 15 Date 12 10 06 Team meeting 10 on Mon 10 30 at 2 30 pm in ECB Advisor meeting 8 on Mon 10 30 at 3 30 pm near 2154 ECB Work on Design Team meeting 11 on Fri 11 3 at 12 00 pm in ECB Team meeting 12 on Mon 11 6 at 2 30 pm in ECB Advisor meeting 9 on Mon 11 6 at 3 30 pm near 2154 ECB Work on Design Lecture Machine Shop required lecture Team meeting 13 on Sat 11 11 at 4 30 pm in ECB Meeting w Amy on Mon 11 13 at 12 pm at Rennebohm Hall Advisor meeting 10 on Mon 11 13 at 3 30 pm near 2154 ECB Work on Design Lab time Diacrylation of PEG from 11 17 11 19 at Rennebohm Hall 12 13 14 15 primarily carried out by Amy Graduate Student from Dr Kao s Lab Work on Design THANKSGIVING Advisor meeting 11 on Mon 11 27 at 3 30 pm near 2154 ECB Lab time Attempted photopolymerization using PEGdA synthesized in Dr Kao s Lab Lab time Diacrylation of PEGdA in Chemistry Lab Lab time Diacrylation of PEGdA in Chemistry Lab Lab time Drying and …