Lab 3: ChemotaxisPost-lab Assignment (10 points)To be submitted electronically by email to your TA as a group at the END of lab. Grading template (completed by TA)Out of Actual PointsTA NotesQuestion 1 1 pointQuestion 2 0.5 pointQuestion 3 1 pointQuestion 4 2 pointQuestion 5 1 pointQuestion 6 0.5 pointQuestion 7 2 pointQuestion 8 1 pointQuestion 9 1 pointTotal 10 ptsExperiment 1 – Mystery wormsPURPOSE: An undergraduate teaching assistant accidentally mislabeled the plates containing the mutant and wild-type worms for today’s experiment. In order to confirm which strain of worm is which, you will design and conduct a controlled experiment to test the preference of mutant (che-2) and wild-type C. elegans to known odorants. 1. What counting rules or strategies did you devise as a group prior to counting worms on your plates? (1 point)In order to count the worms, we will place the slides under the dissecting microscope and count the worms on the attractant/repellent/mystery side first and then move the plate over and count the worms on the water side. Each of us will count the worms on both sideto ensure better accuracy. We also have an idea of how many worms there should be, so we will be able to use that as a marker to insure we’re in an appropriate range. 2. Use the raw data table below to record your data. (0.5 points)Worm strain(A or B)Odorant Worm count (water side) Worm count (odorant side)Wild Type Penny 4 2Wild Type Known attractant 3 2Che-2 Penny 9 3Che-2 Known attractant 9 33. For each strain of worm, predict what the chemotaxis index should be for the positive (0.5 1points) and negative controls. (0.5 points)Che-2 C-elegans: Penny: 0Attractant: 0Wild-type C-elegans-Penny: -.7Attractant: .7Mystery: .64. Calculate the chemotaxis index for each of the tested odorants. Provide a graph of the data. (2 points)X5. Using your data, explain the identities of the mystery strains. (1 point)Experiment 2 – Mystery odorantsPURPOSE: Now that you have determined which plate is which, you will design and conduct a controlled experiment to test the preference of wild-type C. elegans to several mystery odorants. From the data, you will predict the identity of the mystery odorants.6. Use the raw data table below to record your data. (0.5 points)Odorant Chemotaxis Index28 .45 .4293 -.0767 .27. Calculate the chemotaxis index for each of the mystery odorants. Provide a graph of the data. (2 points)Mys#8 Mys#5 Mys#3 Mys#7-0.2-0.100.10.20.30.40.5Mystery Oderant for Wild Type WormChemotaxis INdex8. Rank the mystery odorants from most appealing to least appealing using its chemotaxis index. What are potential identities of the mystery odorants? (1 point)The most appealing odorant was mystery odor number 5, then number 8, then 7, and lastly number 3. Mystery odorant number five was possibly a strong attractant like a ketone. Number 8 could have been methanol, number 7 an amine, and number 3 was the least strong so possibly sodium chloride. 9. Explain 2-3 potential limitations of the experiment. (1 point) A potential limitation of the experiment could be the limited number of repellents/attractants used, since we only used one known attractant, one known repellentand one mystery substance, we can’t definitively determine the extent to which the wild type worms are attracted and/or repelled by the substances. For example, the worms maybe more strongly repelled or attracted by scents other than the ones we used, these scents may serve as a better control than the ones we used. Additionally, the pennies we used as a repellent may not be as effective as they could be, considering pennies are no longer made purely from copper, we don’t know for certain that the ones we used contained a high enough amount of copper. A final limitation could be the small populationof c-elegans worms used, had we added more worms to the sample population we would have more accurate data pertaining to the chemotaxis index and how strong of an attractant/repellent a substance is. 3In the subject line of the email to your TA, please include the word ‘Lab3’, followed by an underscore, the first 4 letters of each group member’s last name, another underscore, and your section meeting time – no spaces. Be sure to CC yourself on the email. For exampleWord document: Lab 3_Link_Shap_Hijm_Bone_TuesAM.docxSubject line of email: Lab