Lab 1: Meet the Worms! Post-lab Assignment (10 points)To be submitted electronically by email to your TA as a group at the END of lab. TEAM NAME: GROUP MEMBER NAMES: Grading template (completed by TA)Out of Actual PointsTA NotesQuestion 1 1 pt 1Question 2 2 pts 2Question 3 1 pt 1Question 4 2 pts 2Question 5 2 pts 2Question 6 2 pts 2Total 10 pts 10 Nice work!Video of C. elegans movement 1) Show a short video clip of your strains to your TA. (1 point)Showed the video.2) Do the different strains move the same way? If they differ, how so? Can you hypothesize a physiological or morphological reason why they might move differently? (2 points)We could only see how the mysky strain moved because we killed all the other strain of worms. The mysky worms looked like they were flopping around on the plate, similar to a fish out of water. The worms may have different placement or size of organelles, or different muscles or a different number and size/shape of muscles that influence how they move throughout the plate.Imaging GFP expression in transgenic strains3) Embed your best GFP images of your strains in the space below. (1 point)14) Do your strains show the same intensity of GFP expression? If not, what do you think might explain these intensity differences? (2 points)We could only see one strain so we can’t compare the two intensities, but if they were different it could be caused by how the genes are expressed and where the proteins are mostconcentrated within the worm. If there is more of a certain protein in a worm, the worm will most likely appear to glow brighter. 5) What is your best guess as to which cells/tissues are expressing GFP in your two strains?Can you refer to a specific webpage link in WormBase that supports your hypothesis? (2 points)For the mysky stain we are hypothesizing that the nervous system is being expressed by GFP. We referenced Worm Atlas and our images appear to be the most similar to the worms 2with GFP showing the nervous system. The GFP is expressed throughout the worm and not necessarily concentrated in one location.6) Is the GFP expression "even" in your expressing tissue (suggesting it is cytoplasmic), or isit localized to subcellular regions? Do you think your strains have transcriptional or translational GFP reporter constructs? (2 points)Our GFP expression is even throughout the worm and slightly more concentrated towardsthe head and tail, it looks to be localized to the head ganglia, tail ganglia, and ventral cord.We think our strains have translational GFP because it is localized to the nucleus of the nervous system which follows a sequence around the entire worm. From this we are hypothesizing that the GFP protein is encoded within the mRNA of the DNA. In the subject line of the email to your TA, please include the word ‘Lab1’, followed by an underscore, the first 4 letters of each group member’s last name, another underscore, and your section meeting time – no spaces. Be sure to CC yourself on the email. For exampleWord document: Lab 1_Link_Shap_Hijm_Bone_TuesAM.docxSubject line of email: Lab