Lab 2: Protein translocationPost-lab Assignment (10 points)To be submitted electronically by email to your TA as a group at the END of lab. Grading template (completed by TA)Out of Actual PointsTA NotesQuestion 1 2 pointsQuestion 2 3 pointsQuestion 3 2 pointsQuestion 4 3 pointsTotal 10 pointsExperiment 1 - How will the pattern of DAF-16 expression change over time in daf-16::GFP transgenic C. elegans worms exposed to heat shock stress?1. Qualitatively describe the results from the experiment. (2 points)The pattern of DAF-16 expression changes from being uniform throughout the cytoplasm of the C. elegans worms, to being dispersed and localized in the nuclei. Following dephosphorylation caused by the stressor (36 deg. water), the NLS signal is activated andDAF-16 is translocated into the nuclei of the worm. The longer the worms were submerged in the warm bath the more DAF-16 was expressed and moved into the nucleus.2. Quantitatively describe the results from the experiment by including a graph of your group’s data. (3 points)The nuclear to cytoplasmic ratio changed from 0 at time 0 to .1227 at time 40 to .15 at time 60.Experiment 2 - How will temperature change the rate of DAF-16 nuclear localization in daf-16::GFP transgenic C. elegans worms?13. Qualitatively describe the results from the experiment. (2 points)The greater the magnitude of the stressor (i.e. higher the temperature of water), the greater the rate of translocation of DAF-16 into the nucleus occurred until 36 degrees and then the ratio decreased. As temperature of the water bath that the worms are submergedin increases, the rate at which dephosphorylation of DAF-16 can occur also increases up until an optimal temperature and then it starts to decrease. When the rate of dephosphorylation increases, the expression of the NLS signal is amplified and DAF-16 can translocate into the nucleus via the nuclear pore. 4. Quantitatively describe the results from the experiment by including a graph of the class data. (3 points)The ratio was 0 at 32 degrees celsius and increased to .0434 and then to .1227 at 36 degrees celsius, but once it got to 38 degrees celsius it began to decrease to .0865. We are hypothesizing that past 36 degrees the worms are beginning to be harmed and can’t express to DAF-16 as they previously did and therefore dephosphorylation cannot occur as it did at lower temperatures. In the subject line of the email to your TA, please include the word ‘Lab2’, followed by an underscore, the first 4 letters of each group member’s last name, another underscore, and your section meeting time – no spaces. Be sure to CC yourself on the email. For exampleWord document: Lab 2_Link_Shap_Hijm_Bone_TuesAM.docxSubject line of email: Lab