Review 3 1 Match the general type of biochemical reaction catalyzed in the left column with the class of enzyme listed in the column on the right removes a phosphate group from a molecule A ATPase hydrolyzes ATP B polymerase hydrolyzes bonds between nucleotides C ligase adds phosphate groups to molecules D kinase catalyzes reactions in which one molecule is E isomerase oxidized and another is reduced F nuclease hydrolyzes peptide bonds G oxido reductase joins two ends of DNA together H protease catalyzes the synthesis of polymers such as I phosphatase RNA and DNA rearranges bonds within a single molecule 2 Any substance that will bind to a protein is known as its Enzymes bind their at the Enzymes catalyze a chemical reaction by lowering the because they provide conditions favorable for the formation of a intermediate called the Once the reaction is completed the enzyme releases the of the reaction activation energy inhibitors substrates free energy ligand products active site transition state high energy isomers low energy 3 The active site of an enzyme usually occupies only a small fraction of the enzyme surface True or False 4 Catalysis by some enzymes involves the formation of a covalent bond between an amino acid side chain and a substrate molecule True or False 5 Allosteric enzymes have two or more binding sites True or False 6 The specificity of an antibody molecule is contained exclusively in loops on the surface of the folded light chain domain True or False 7 Affinity chromatography separates molecules according to their intrinsic charge True or False 8 Feedback inhibition M N 1 O 2 P 3 Q 4 R 5 1 The final product R will most likely inhibit which reaction a 1 b 2 c 3 d 4 e 5 2 Which two enzymes would be the most likely ones to regulate if this pathway is freely reversible and can go both ways a 1 and 2 b 1 and 3 c 1 and 5 d 2 and 4 e 4 and 5 9 Fill in the blanks with the labels in the list below to identify various parts of the antibody structure in Figure A B C D E constant domain of the light chain constant domain of the heavy chain antigen binding site variable domain of the heavy chain variable domain of the light chain 10 The human immune system produces of different immunoglobulins also called which enable the immune system to recognize and fight germs by specifically binding one or a few related The hypervariable structural element that forms the ligand binding site is comprised of several Purified antibodies are useful for a variety of experimental purposes including protein purification using chromatography affinity antibodies antigens strands 11 billions coiled coils hundreds ion exchange ligands loops size exclusion Regulation of protein function Amount of protein Location of protein Modification of protein activity by modifying the protein s shape o feedback inhibition of allosteric proteins o positive regulation of allosteric proteins o protein phosphorylation o other covalent modifications of proteins o binding of GTP o binding and hydrolysis of ATP
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