GENE 412 1st Edition Lecture 4 Outline of Last Lecture I X2 Chi Squared Test II P 05 Concludes Outline of Current Lecture I Gametic Phase Linkage Equilibrium II Things Population geneticists like Current Lecture I Gametic Phase a Two Locus H W i CAT CAT CAT CAT GAT CAT CAT CAT CAT CAT CAT CAA Last row shows Two Locus H W because recombination or crossover must occur to get back to normal genotype b Genetic Polymorphism i Presence of 2 or more alleles at 5 or 1 1 1 recombination 1 106 nucleotides 2 recombination does not happen too often when genes are close together AB ab AB AB AB AB ab ab AB ab ab ab c Ab aB recombination is only way to get this genotype i AB ab Coupling ii Ab aB Replusion d D is the percentage of recombination i D f AB f ab f Ab f aB ii If D 0 then Dmax is lesser of f Ab or f aB iii If D 0 then Dmax is lesser of f AB or f ab These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute II iv D D Dmax v Best marker for recombination is the largest D Things population geneticists like a Like lots of alleles because that means a lot of information b Codominant or incomplete dominant c Like to indentify alleles with confidence don t confuse two genes with two alleles of one gene d Ways to look at data i Microsatellite Analysis 1 LOOKING FOR MOBILITY 2 Different copy number can give you a different number of alleles ii Mitochondrial DNA analysis 1 Inherited mother to opffspring 2 Take amplified piece with restriction enzyme to determine if DNA has a particular sequence will cut DNA if sequence is present iii Randomly Amplified Sequences of DNA RAPID s 1 Get amplified product but can t tell if two products alleles or two completely unrelated alleles PROFESSOR SAYS HE DOES NOT LIKE THIS