Forensic Biology by Richard LiGeneral ConsiderationsTypes of AntibodiesTitration of AntibodiesSpecificityOptimal ConditionsAssaysSlide 8Chapter 7: Species IdentificationSpecies Identification:In the past, done by serological methodsNow, accomplished by DNA quantitationCan be a good screening method for unnecessary testsCan be used at the crime sceneNecessary in animal casesUse primary and secondary binding assaysForensic Biology by Richard Li 2Antihuman serumAlbumin Hemoglobin antibodyGlycophorin AForensic Biology by Richard Li 3Series of dilutions of antiserum are made and each dilution is then tested for activity using either precipitation or agglutination methodsTiter- the reciprocal of the highest dilution giving a positive reaction.Forensic Biology by Richard Li 4Most antihuman sera usually have cross-reactivity with higher primatesCrimes involving primates are rareForensic Biology by Richard Li 5Proper buffer systemIonic strengthIntro of polymersTemperaturepHForensic Biology by Richard Li 6Immunochromatographic AssaysIdentification of human hemoglobin protein▪Hexagon OBTI▪ABAcard HemaTraceIdentification of human glycophorin A protein▪RSID-BloodForensic Biology by Richard Li 7Precipitation-based assaysRing assayOuchterlony assayIdentityNon-identityPartial identityCrossed-over electrophoresisForensic Biology by Richard Li
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