Forensic Biology by Richard LiBasicsHuman Mitochondrial GenomemtDNA Polymorphic RegionsSlide 5Forensic mtDNA TestingInterpretation of MtDNA ResultsChapter 21: Mitochondrial DNA ProfilingmtDNAUseful tool for human identificationMaternally inheritedNumber of copies of MtDNA genome per cell is higher than nuclearHair shaft, bones, decomposed samplesForensic Biology by Richard Li 2Subcellular organelles which are energy-generating Mitotype10x higher mutation rateRevised Cambridge Reference Sequence (rCRS)16,569 nucleotides and 37 genesControl region (D-Loop)H-Strand and L-StrandForensic Biology by Richard Li 3Located in D-LoopHypervariable regions:HV1HV2HV3Most commonly region in forensics:▪HV1▪HV2Forensic Biology by Richard Li 4HeteroplasmySequence HeteroplasmyLength HeteroplasmyForensic Biology by Richard Li 5Decomposed or skeletal remainsMtDNA screen assayAllele-specific oligonucleotide (ASO) assayLINEAR ARRAY kitmtDNA SequencingPCR amplificationDNA sequencing reactionsSeparation using electrophoresisData collection and sequence analysisForensic Biology by Richard Li 6SWGDAN & ISFGInclusion (match)ExclusionInconclusiveForensic Biology by Richard Li
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