QUIZ 2ECOL/MCB320 Print Name Spring 2007 Sign Name QUIZ 2Instructions: Print and sign your name at the top of the page. Show calculations so we know youactually did the problem rather than guess the answer. In multiple choice questions, circle the letter of the best answer. (Approximate numbers of points are in parentheses.)1. (2) Below is a eukaryotic DNA molecule caught in the act of replicating. Note that there are discontinuous and continuous strands. Letters a-d refer to ends of the molecule. State whether each of the following is a 5’ or a 3’ end:a 3’ c 5’ b 5’ d 3’ 2. (2) A mutation in a gene causes the protein product of the gene to be shortened from 120 to 88 amino acids; in addition, 8 of the last 13 amino acids in the shortened protein differ from those in the normal protein. The best term to describe the mutation is:(a) nonsense mutation(b) missense mutation(c) frameshift mutation(d) deletion mutation3. (2) The restriction enzyme BamHI cuts like this: 5' G|G A T C C3' C C T A G|GSuppose that a plasmid is cut with BamHI. For each of the following enzymes, circle yes if DNAcut with the enzyme can be ligated with the plasmid, or no if not: (a) MboI 5' |G A T C yes no3' C T A G|(b) PvuI 5' C G A T|C G yes no3' G C|T A G C24. (2) Which of the following is a synonymous substitution (in a sense strand)?(a) ACT -> AAT(b) GCT -> GAT(c) TCA -> TAA(d) GGA -> GGG5. (2) Write the base sequence of the anticodon for the GAA codon:5’ UUC 3’THE (ALMOST) UNIVERSAL GENETIC CODE U C A G UUU phe F UCU ser S UAU tyr Y UGU cys C UU UUC phe F UCC ser S UAC tyr Y UGC cys C CUUA leu L UCA ser S UAA och Z UGA opa Z AUUG leu L UCG ser S UAG amb Z UGG trp W G CUU leu L CCU pro P CAU his H CGU arg R UC CUC leu L CCC pro P CAC his H CGC arg R CCUA leu L CCA pro P CAA gln Q CGA arg R ACUG leu L CCG pro P CAG gln Q CGG arg R G AUU ile I ACU thr T AAU asn N AGU ser S UA AUC ile I ACC thr T AAC asn N AGC arg R CAUA ile I ACA thr T AAA lys K AGA arg R AAUG met M ACG thr T AAG lys K AGG arg R G GUU val V GCU ala A GAU asp D GGU gly G UG GUC val V GCC ala A GAC asp D GGC gly G CGUA val V GCA ala A GAA glu E GGA gly G AGUG val V GCG ala A GAG glu E GGG gly G G
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