UIUC MCB 450 - mcb450 lecture 1 (42 pages)

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mcb450 lecture 1



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mcb450 lecture 1

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Pages:
42
School:
University of Illinois at Urbana, Champaign
Course:
Mcb 450 - Introductory Biochemistry
Introductory Biochemistry Documents

Unformatted text preview:

7 1 MCB 450 Lecture 7 O Glycoconjugates Proteoglycans Glycoproteins Glycan Synthesis Examples of Importance of Glycans 7 2 Glycoconjugates NOTE THE MODIFICATIONS WITH SUGARS ARE ALL ON EXTRACYTOPLASMIC PORTIONS OF PROTEINS AND LIPIDS PROTEIN 7 3 Definitions proteoglycan vs glycoprotein Proteoglycan Protein Uronic acid and or SO42 containing polysaccharide glycosaminoglycan Protein Glycosaminoglycan GAG Glycoprotein Protein Oligosaccharide not GAG covalently attached to peptide backbone 7 4 Glycosaminoglycan repeats 1 One sugar always GalNAc GlcNAc or GlcN often esterified with sulfate The other usually a uronic acid e g glucuronate GlcA GalNAc 7 5 Glycosaminoglycan repeats 2 Ionized carboxyl sulfate groups give molecule a ve charge Assume e x t e n d e d c o n f o r m a t i o n in solution Repeating disaccharide HYALURONIC ACID disaccharide repeats chain GlcA 50 000 GlcNAc CHONDROITIN SULFATE GlcA GalNAc KERATAN SULFATE GlcNAc Gal 20 60 25 7 6 Glycosaminoglycans in vertebrates HYALURONIC ACID CHONDROITIN SULFATE KERATAN SULFATE Highly hydrated Forms viscous solution is lubricant in joints also structural component of extracellular matrix of vertebrate cells In cartilage tendons ligaments aorta walls No uronic acid In cartilage bone nails horn hoofs 7 7 Cartilage proteoglycan Central filament of hyaluronate SUPRAMOLECULAR COMPLEX OF GLYCOSAMINOGLYCAN CHAINS PROTEINS Aggrecan Electron micrograph Mass 2 x 106 daltons 7 8 Cartilage proteoglycan Can spring back after being deformed so cartilage can cushion compressive forces HIGHLY HYDRATED 7 9 Definitions proteoglycan vs glycoprotein Proteoglycan Protein Uronic acid and or SO42 containing oligosaccharide glycosaminoglycan Protein Glycosaminoglycan Glycoprotein Protein Oligosaccharide not GAG covalently attached to peptide backbone Two major types of linkage to extracytoplasmic domains of proteins Occur with variations in all eukaryotes Examples in bacteria and archaea 7 10 Two major types of protein saccharide linkage



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