Lab Images Friday September 18 2015 12 57 AM Lab 1 Termite flagellate 400x What type of microscope is used to view minute organisms such as these flagellates from a termite s gut Compound microscope Dissecting microscope stereoscopic zoom microscope What type of scope is shown Is it used for larger or smaller items Dissecting or stereomicroscope Used for larger specimens Compound microscope light housing and iris diaphragm Identify the iris diaphragm What type of microscope is shown Compound scope Compound microscope nosepiece objectives stage Identify objectives stage nosepiece slide holder What type of microscope is shown Compound microscope Letter e w m As magnification increases 100x the amount of light needed to view the specimen and the field of view becomes Increases smaller Crossed threads In microscopy as magnification increases depth of field How do you adjust the focal plane at high magnification Decreases with the fine focus ONLY Lab 2 Black ink chromatography separation of pigments in black ink by water chromatography In this demonstration what is the solvent and what is the solute Solvent water Solutes pigment particles in the ink Lugol positive test for starch right test tube using Lugol s solution What type of carbohydrates does testing with Lugol s solution detect Starch or specifically amylase Osmotic Pressure blood cells effects of tonicity on red blood cells The process by which cells lose water in a hypertonic solution is Plasmolysis Red cabbage pigment Which has a higher solution at different pH concentration of H ions an levels acidic or basic solution Acidic Water strider demonstrating surface tension of water What property of water allows these insects to walk on its surface High surface tension Human Erythrocytes Osmotic Pressure red blood cells in hypertonic isotonic hypotonic solutions In a hypertonic solution water is moving out of the cells Elodea hydrophytic leaf Anacharis leaf 400x Are these plant or animal cells Identify some of the structures Plant nucleus chloroplasts cell wall vacuole Elodea hydrophytic leaf whorled leaf arrangement Why is Elodea Anacharis a commonly placed in fresh water aquaria It releases oxygen as a product of photosynthesis which is essential to keep fish healthy Elodea hydrophytic leaf w m 100x chloroplasts cytoplasmic streaming Name two ways the green dots support life on earth Site of photosynthesis which produces O2 and sugar O2 is essential for aerobic respiration Sugar is basis of most food webs Close up of Elodea showing chloroplasts and central vacuole What is the evolutionary history of chloroplasts in plant cells Believed to be originally cyanobacterial endosymbionts Lab 3 Three indicators for biomolecules What does a positive test with each of these three reagents indicate Benedict s reducing sugar Lugol s starch Biuret peptide bonds proteins Benedict s test for reducing sugars What color is a negative test with Benedict s solution Negative pale blue What color is positive Positive depends on the amount of sugars present at high concentrations and with heat a positive Lugol positive test for starch right test tube using Lugol s solution What type of carbohydrates does testing with Lugol s solution detect Starch or specifically amylase Positive Biuret test for peptide links protein Organic Molecules Tests A Negative Biuret B Positive Lugol s C Positive ninhydrin D Positive Benedict s E Negative Benedict s In the presence of peptide bonds Biuret reagent changes from light blue to purple Which of these tests shows the presence of an amino acid Positve ninhydrin Ninhydrin What does this indicator test for and what color denotes a positive result Amino acid purple or yellow Aldehyde What type of molecule is this The H C O group is terminal so this is an aldehyde Onion epidermal peel with negative Lugol s stain Based upon this Lugol s test is starch stored in onion cells No Ketone What sort of compound is shown Ketone the C O is in the middle of the molecule Carboxyl group What functional group is present in this molecule Carboxyl OH C O Amine compound What sort of chemical compound is this Amine the nitrogen is a giveaway Positve Sudan IV Test In lab the positive Sudan IV test indicates the presence of what type of biomolecule Lipid Functional Groups of Organic Molecules A ketone B amine C aldehyde D carboxyl E hydroxyl Identify the functional groups empty sticks indicate the location of the rest of the molecule A ketone B amine C aldehyde D carboxyl E hydroxl Lab 4 Spectrophotometer Identify sample compartment wavelength selector mode selection screen where results appear Spectrophotometer diagram Refer to the lab manual and identify the labeled structures Lab 5 Gram stain reagents What does each of these reagents do in a Gram staining procedure What other reagent is needed Crystal violet stains peptidoglycan Iodine fixes violet Alcohol decolorizes by removing outer layer Reagents used in Gram staining in scrambled order In what order are these used answer violet water iodine water alcohol water safranin water Paramecium w m 100x Identify this organism How does it move Paramecium cilia Human sperm spermatozoa stained 1000x Describe how the form of these cells fit their function Mobile sperm cells must swim to the egg flagella propels the nuclear material forward Squamous epithelium isolated cells Is this an animal or a plant cell How can you tell human male 1000x Animal human no cell wall Squamous epithelium isolated cells Identify the large dark mass in the center of the cell human male 1000x Nucleus Plant animal and generalized cell models What is the central empty feature in the leftmost cell Is this a plant or animal cell Vacuole plant Animal Cell Identify plasma membrane nucleus Golgi apparatus ribosome mitochondrion centrioles flagellum Escherichia coli bacillus gram negative bacteria prokaryote 1000x To what domain do these organisms belong Bacteria Escherichia coli bacillus gram negative bacteria prokaryote antibiotic plate Which antibiotic appears to be most effective against the bacterial culture in this test plate How can you tell C It has the largest zone of inhibition Bacteria flagella w m 1000x What is the function of bacteria flagella Locomotion 3 bacteria Bacilli Spirilla and cocci 1000x What is the name for the spiral shaped bacterium Spirillum Bacillus w m 1000x Describe the characteristic shape of these organisms Bacillus or bacilli Bacillus subtilis prokaryote 1000x gram positive bacteria What