Instructor Caleb Rounds Exam III Biology 151C Name yours Directions For questions with lettered answers choose one For numbered answers choose A for true B for false and C E for please mark this wrong 1 I have correctly entered my valid student ID and name on the answer sheet and I am currently enrolled in Caleb Rounds Bio151C class A Yes I have am B Who wants to know 2 A n reaction is spontaneous an one is not A B C D E Exergonic endergonic Endergonic exergonic Exothermic energetic Endothermic exothermic Both B and D are correct Regarding the chart above A is true B is false 3 The reaction at left is endergonic B 4 An enzyme could lower the G of the reaction on the left B 5 The reaction at right could be coupled to the formation of ATP to make the overall reaction exergonic B 6 The energy released in the reaction at left cannot be used to do work like pumping protons across a membrane it will be lost as heat B 7 Alpha tocopherol is an anti oxidant usually called Vitamin E Which of the following could act to recycle tocopherol so that it could continue to act as an anti oxidant assume appropriate redox potential A B C D E Reduced vitamin C ascorbic acid Oxidized vitamin C ascorbate NADP NAD ATP 1 Instructor Caleb Rounds Exam III Biology 151C 8 GTP GDP Pi is catalyzed by GTPases like RAS GTPases are important regulatory molecules that are used to control many different reactions in the cell The reaction is very similar to ATP ADP Pi energetically How would the concentration of GDP in the cell affect the actual free energy of the reaction in the cell not the standard free energy A B C D E It would not If there were a lot of GDP relative to GTP it would lower the G make it more negative If there were very little GDP relative to GTP it would lower the G make it more negative It is impossible to tell from the given information The reaction is endergonic and needs the enzyme to make it happen therefore the concentration of the GDP is irrelevant Complex I of the electron transport chain in mitochondria is known as NADH dehydrogenase It A is true B is false 9 10 11 12 13 14 Reduces NADH B Oxidizes NAD B Is in the inner membrane of the mitochondria A Is only in animals B Oxidizes NADH A Accepts an electron from NADH A Enzymes A is true B is false 15 16 17 18 Can make an endergonic reaction exergonic B Lower the activation energy of a reaction A Do not occur in fungi B Are usually proteins A Two enzymes smileyface hydrogenase km 1 6 M Vmax 25 M and poutyfase reductase km 5 M Vmax 25 M can both convert the substrate pouty smiley using NADH Vmax should have read 25 mole s 19 Poutyface reductase should work better have a higher rate than smileyface hydrogenase at low pouty concentrations B 20 At high concentrations of pouty both enzymes will have the same rate A 21 At 2 M poutyface reductase will be saturated B 22 What is the proper order of these steps starting from glucose A B C D Pyruvate glycolysis ATP synthase Citric acid cycle pyruvate ATP synthase Glycolysis citric acids cycle chemiosmotic ATP production Citric acid cycle chemiosmotic ATP production pyruvate 2 Instructor Caleb Rounds Exam III Biology 151C E Glucose pyruvate Calvin cycle A B C D In the above diagram question 23 D represents an uncatalyzed exergonic reaction no enzyme present whereas question 24 B represents the same reaction with an enzyme present Use the above diagram for the next 5 questions The fake data above could be for an enzyme that catalyzes the conversion of arachidonic acid to prostaglandin leading to inflammation and pain Curve A represents the uninhibited enzyme the other two curves represent the effect of two drugs on that enzyme 3 Instructor Caleb Rounds Exam III Biology 151C 25 B is probably the result of a competitive inhibitor 26 The Km of A is roughly A B C D 5 M 15 M 40 M Impossible to determine from the given information 27 The Vmax of A is achieved at roughly A B C D 5 M 15 M 40 M Impossible to determine from the given information 28 If a patient s arachidonic acid level is 10 M which drug should she take A B C D drugs are bad or E this joke s getting old 29 Which drug binds to a site other than the active site on the enzyme A B C D B A D C Use this chart to answer the next 3 questions 30 A should be used to determine the initial velocity 31 Circle B is Vmax A is true B is false 4 Instructor Caleb Rounds Exam III Biology 151C 32 The enzyme used in this chart is saturated at A 30 mM B 50 mM C 60 mM D 70 mM E Do I look like an enzymologist 33 If you were to equilibrate mitochondria and chloroplasts in a solution of pH 7 0 until all of the compartments were the same then switch them to a pH 6 5 solution with ADP and Pi until it equilibrated with the intermembrane space of mitochondria and the stroma of chloroplasts what might happen A B C D E Total chaos The mitochondria would make ATP The mitochondria would use ATP to make ADP and pump protons The chloroplast would make ATP The chloroplast would use ATP to make ADP and pump protons Use the glycolysis chart above for the next four questions 34 If you were to add glucose and phosphate to a test tube to produce glucose 6 phosphate would you predict the reaction would be exergonic or endergonic Use information from the chart A Exergonic B Endergonic C Impossible to predict 5 Instructor Caleb Rounds Exam III Biology 151C D It depends on the pH 35 In the absence of NADH glycolysis A B C D E Would not be inhibited Would need fermentation to generate more NADH Would rely on ATP Would be unable to reduce glyceraldehyde 3 phosphate A and B are correct 36 14C labeled fructose can enter glycolysis by being phosphorylated to fructose 6 phosphate but it will not make as much ATP as glucose A is true B is false 37 Glycolysis can proceed in the absence of ATP A is true B is false 38 All of the CO2 we exhale comes from the citric acid cycle A is true B is false 39 Erythrocytes are red blood cells they do not have mitochondria Do they use more or less glucose to make the same amount of ATP as a muscle cell that has mitochondria and oxygen A B C D More Less Same Impossible to determine 40 In the reaction above pyruvate A B C D Reduces NAD Oxidizes NAD Reduces Acetyl CoA Oxidizes Acetyl CoA 41 What output of the citric acid …