Lecture 20– Oct 17Chem 4101 – Fall 2008Mass Spectrometry1- Mass Spectrometer Components2- Atomization/Ionization3- Mass analyzers-Chapters 11, 20Lecture 20– Oct 17Chem 4101 – Fall 2008Mass Spectrometer ComponentsFigure 11-1, 20-11, Sections 11A, 20CLecture 20– Oct 17Chem 4101 – Fall 2008Ion Sources – Electrospray ionization (ESI)Figure 20-9, Section 20CLecture 20– Oct 17Chem 4101 – Fall 2008Lecture 20– Oct 17Chem 4101 – Fall 2008Lecture 20– Oct 17Chem 4101 – Fall 2008ESI-MS http://aston.chem.purdue.edu/files/u2/desi2.pngLecture 20– Oct 17Chem 4101 – Fall 2008Matrix-assisted laser desorption ionization (MALDI) Figure 20-7, Section 20BLecture 20– Oct 17Chem 4101 – Fall 2008Section 20BMALDI matricesLecture 20– Oct 17Chem 4101 – Fall 2008MALDI-TOF MS spectrumFigure 20-8, Section 20BLecture 20– Oct 17Chem 4101 – Fall 2008Mass Analyzer- Time of FlightFigure 20-15, 11-10 Section 20B, 11BLecture 20– Oct 17Chem 4101 – Fall 2008Mass Analyzer- Time of FlightFigure 20-15, 11-10 Section 20B, 11BLecture 20– Oct 17Chem 4101 – Fall 2008Mass Analyzer- QuadrupoleFigure 11-6, Section 11BOn your own, do simulation suggested in the textbook (page 289)Lecture 20– Oct 17Chem 4101 – Fall 2008Quadrupole- Voltage relationships to make an m/z scanFigure 11-9, Section 11BLecture 20– Oct 17Chem 4101 – Fall 2008Fourier Transform SpectrometersFigure 11-9, Section 11BLecture 20– Oct 17Chem 4101 – Fall 2008FT-MS excitation and signalFigure 20-19, Section 20CLecture 20– Oct 17Chem 4101 – Fall 20081,1,1,2-tetrachloroethane spectrumFigure 20-20, Section 20CWhat is the resolution in this measurement?Lecture 20– Oct 17Chem 4101 – Fall 2008Tandem MS (MS/MS) Figure 20-21, Section 20CLecture 20– Oct 17Chem 4101 – Fall 2008Lecture 20– Oct 17Chem 4101 – Fall 2008Tandem MS of precursor ion 279 m/zFigure 20-22, Section 20CWhat fragments correspond to the detected ions?Lecture 20– Oct 17Chem 4101 – Fall 2008Summary 1. Two common ionization method are electrospray ionization and matrix-assisted laser desorption ionization. Both are soft ionization methods.2. Mass analyzers include time-of-flight tubes, quadrupoles, and ion traps.3. Tandem mass spectrometry provides both MW and structural information. The collision mode determines the types of fragments that will be produced (product ion spectra) from the precursor
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