10/10/09 1 Lecture 13 – Oct 12 Chem 4101 – Fall 2009 Fluorescence 1- Fluorescence and phosphorescence Theory 2- Excitation and emission spectra 3- Molecular features of fluorophores 4- Instrumentation -Sections 15A – 15B Suggested exercises: 15-1, 15-2, 15-3, 15-4 Lecture 13 – Oct 12 Chem 4101 – Fall 2009 Energy diagram – Jablonski diagram Figure 15-2, Section 15A Lecture 13 – Oct 12 Chem 4101 – Fall 2009 Excitation and emission spectra for quinine Figure 15-3, Section 15A10/10/09 2 Lecture 13 – Oct 12 Chem 4101 – Fall 2009 Molecular rigidity – favors fluorescence think, π π* Section 15A Φ = 1.0 Φ = 0.2 Lecture 13 – Oct 12 Chem 4101 – Fall 2009 pH Effect Section 15A High pH, more stable first excited state, more fluorescent Low pH low fluorescence Lecture 13 – Oct 12 Chem 4101 – Fall 2009 Fluorescent (Fluorophores) and non-fluorescent molecules Figure 7-4, Section 7B10/10/09 3 Lecture 13 – Oct 12 Chem 4101 – Fall 2009 Molecular features and fluorescence Section 15A Lecture 13 – Oct 12 Chem 4101 – Fall 2009 pH Effect Section 15A Lecture 13 – Oct 12 Chem 4101 – Fall 2009 Fluorometer Figure 15-10, Section 15B10/10/09 4 Lecture 13 – Oct 12 Chem 4101 – Fall 2009 Spectrofluorometer Figure 15-11, Section 15B Lecture 13 – Oct 12 Chem 4101 – Fall 2009 Instrument Standarization Figure 15-14, Section 15B 10 µM Quinine sulfate, 0.2 M
View Full Document
Unlocking...