Slide 1BackgroundsAnalytesHypothesisReverse-Phase HPLC MSInstrumentationSample preparationResultsMethod specifications:Alternative methodsMethods for other comparisonsReferences:Comparative Analysis of Arbutin and Tranexamic Acid in Skin Whitening ProductsChuxin Chen12-07-2011Backgrounds•According to the aesthetic of most estern Asian people, women are more attractive in brighter skin tones. Thus the skin whitening products have a large market in countries like Japan, Korea and China.•Different types of whitening products varied a lot in prices, I would especially investigate in how the tranexamic acid added products are different form the arbutin added products.AnalytesHypothesis•The hypothesis is: the tranexamic acid added products are more effective, stable and healthier than the arbutin added products.Reverse-Phase HPLC MS•The reverse-phase HPLC will give good peak resolutions and high efficiency coupled to tandem MS•Able to quantify analytes by using a internal standard solution.Sample reservoirPump to produce high oressureSample injectionColumn tubeion sourcemass analyzerdetectorData systemInstrumentation•HPLC: Agilent 1200•Column: Nucleosil brand C18 (10µm,25*0.46cm)•The mobile phase for the separation was acetonitrile/water (v:v 50:50), the PH of the mobile phase was adjusted to 2.6 by phosphoric acid (85%, w/w).•Detector: tandem MSSample preparation •10ml of the internal standard solution (0.5g/L) is made and added into the 100µL sample which was contained into a 1.5mL plastic tube. •100µL of perchloric acid(2.5% w/w) were than added.•sample was then vortex mixed for 30s and centrifuged at 14,000rpm for 10min.•100µL of the aqueous supernatant is decanted into another tube and 150µL of sodium hydroxide (0.1M) was added.•sample is vortex mixed for 10s, and transferred into injection vials for analysis.ResultsMS of tranexamic acid derivative of Ethyl chloroformateMS of arbutin transfer product by rBglAMethod specifications:•Concentration range: 1.0-200.0 µg/mL• linear regression correlation coefficient: 0.9991•CV range: 0.7-8.6%•LOD: 0.05 µg/mL•LLOQ: 1.0 µg/mL•ULOQ : 200.0 µg/mL•Accuracy: 96.9%Alternative methodsMethod Advantage Disadvantage Capillary Zone Electrophoresis (CZE)Can have a good separation of analytes, fast rate. Too expensiveHPLC UV-Vis UV-Vis detector can detect and quantify analytes wellAnalytes are not able to be recognized since the overlap of the organic species in certain regions.Methods for other comparisonsEffectiveness: 100 volunteers, both will keep apply the arbutin added skin supplies on the left wrist and tranexamic acid added skin supplies on the right wrist everyday, the brightness effect of skintone can be compared after 2 months’ experiment.Stability: >analytes will be exposed in the open air, keep checking by NMR which one is oxidized by air or decompose first.>analytes will be exposed under light, keep checking by NMR to see if any of them is light reactive.References:•No time left….. ( >>>
View Full Document