';"ln i+MIC 301 Third ExamMarch 28, 2005This examination will be scored by machine. One answer sheet will be supplied to eachstudent. Record your llAgg ard Student Number on the ansryer sheet. Check to be certainthat you have a total of 40 questions.You !qq!l! use an ordinary #2 lead pencil to enter your answ€rs on the answer sheet. Be sure thatyouf marks are heavy and black. ERASE COMPLETELY ary answer you wish to change.There is only gNE corect mark for each answer. TEE ]\NSWER NUMBER ON THEANSWER SHEET MUST CORRESPOND WITH THE OUESTION ON THEEXAMINATION.At the end ofthe examinatioL students may keep the questions. Answers will be posted by 2:00p.m, on Blackboard.Challenges to questions may be submitted !4_II!!i!g to 261 BEB with appropriate justificationno later than 2:00, Nlarch 30,2005.SECTION A: Each of the statements below is followed by five suggested correct answers orcompletions. Select the QNE that is !9!l! in each case, and then blacken the appropriate circle onthe answer sheet.1. All of the following are corect for members of the genus Mycobacterium EIgEgf2.3.A. they contain a higher proportion of lipid than other bactedalB. they are not able to be Gram stained iC. they have a long gereration time of12 20ho rs ID. they are more rcsistant than other pathogenic bacteia to disinfectants and stains -1E,l they are scnsitive to common antibaclerial antibioticsNeisseia meningitis can be distinguished unequivocally from i/elss ei.t go orrhoeae rntheclinical laboratory by the\. Gram staining reaction,8, presence ofpili (fimbriae) on the cell surfacey$ ability to make a protease that degrades IgA antibodies'!. presence ofpeptidoglycan in its cell wallE; production of acid by-products dudng metabolism of the sugars glucose andllqllsseAII of the following are conect of leprcmatous leprosy E&gIA. clinical folm accountable for disease associated disfiguration ;& chronic disease ofskin and lerves Irqj usually diagnosed by cultue on the selective medium Lowenstein-JensenD. lesion locations (lepromas) are the cooler parts ofthe bodyE. neurological evaluation of anesthesia in the lield includes the "feather test" ,,t r .-li .--.,-,,'State University ofNe$ York rl BuffaloDepartnent of MicrobiologyLMIC 301 Third ExamMarch 28, 2005State University ofNew YorkDepartmel1t of Microbiology].,)SECTION A (con't)4. All ofthe following are correct for a positive tuberculin skin test EIgqgIC it is an indicator of a rcceltulirect couse inlbction onlyB. it can be induced by vaccination with the ' Bacille Calmet-Gurein 'C. it ploduces an induation of (> l0 nrm) rvith erythema and edemaD. it indicates pdor contact with mycobacterial proteinsE. tuberculin €vokes a delayed hwersensitivity reaction at the site5. Obseryation of Gram negativg, coffee bean-shaped, diplococci during microscopicexamination of a specimen of celgbral spinal.fhid is presumplive evidence of whichbactedumA. Streptococcus pneumoniaeB. Salmonella typhiQ. Nelsseia gohothoeaeu) l\elssena me4 t4glt1rtsE. Streptococcus mutansofthe following are obligate human palhogens EXCEPTA. Sa Imon e I I a en teriditisJ-,)t\satnonetta UPnlt. Q Shigella flexneriD. Yibrio cholerae-,t naemopnuus I nJtuenzael,-/W e rn op hi lus du cr e y i!!/produces an erzlane which degades lgc antibodies in the plasma (semm)B. binds to the surface of gut (intestinal) cells using a flagellumC. causes oritis media by dissemination from Lhe intected gutD. has been responsible for seven worldwide pandemicsE. is associated with increased chances ofconhactins HIVWhich serot)?e oflldemophilus influenzae is gllgl[y the predominating disease-causingform in the United States?,A\Serogloup A (HiA)a B/Serogoup B (HiB)C. Sero$oup C (HiC)D. Serogroup D (HiD)E. Non-tpeable CNTHI)Al1MIC 301 Third ExamMarch 28,2005State University ofNew YorkDepartment of MicrobiologySECTION A (con't)9. Which ofthe following is characteristic of Streptococcus pyogenes?! Gram negative coccusPi, growth inhibiled by bacitracir.C. organism is lysed by the action ofbile saltsD. lipooiigosaccharide toxin is present in its cell wallE. capable of fermenting sugars to produce lactic acid that causes dental canesf!. Wlrich of the following is charactedstic of Streptococcus p eufioniae?A,r causes beta-henolysis u,hen cultured on blood agar platesB. a common cause ofimpetigoC. produces a polysaccharide capsule that hinders phagocl'tosisp* Brows well in laboratory medium containing 6.5% NaClE;. produces a protein exotoxin (SpeA) that causes scarlet fever11. The pathogenic bacteium, Neisseria gonorrhoeae is kJtowri'toA. survive for long periods orltside the host on cnvironnental surfacesB. cause disease in a variety ofanimalsA $ow rapidly on laboratory medium at room temperatureiD/ colonize the mucus membranes ofthe hostE. possess numercus flagella12. All ofthe following statements are correct conccming the nomal microbiola EIc!3IA. micrcbes begin to colonize body surfaces at the time of birth ,md continue to, . colonize thrcughout our lifetime -li B. it contains collrmensal microorganisms that never cause disease iC. some members are hansie[t, being present for a time and then disappear iD. the normal microbiota contains species of Staplrylococats and StreptococcusE. the composition ofthe microbiota is differe[t in each person I\i\-t?)f--".15. The tissue lesion that is characteristic ofdiseases caused by Saphylococttrs aureus si"! bacteria and pus dispersed along fascia planes between muscle and skin tissueB. an accumulation ofbacteria and pus coniined in a tissue cavity (pocket)C. petechial hemonhages in the skinD. er)thema of the posterior phar)'!x with whitish exudates and enlarged regiolall)T nph nodesE. a diffuse rcd rash ofthe skinMIC 301 Third ExamMarch 28, 2005State University ofNew YorkDepartment of Micrcbiology. SECTION A (con't)i-*d Lipooligosaccharide. IgA protease, soluble peptidoglycan fragrnents, fimbriae (pili) and' : polysaccharide capsule are all virulence factors ofwhich bacterium?ANeis s elid meningiditisStrept o coca8 a galacliaeEnterococcus faecalisEscherichia coliYibrio cholerae15. All ofthe following statements are gg4991E conceming pelvic inflammatory disease (PID)caused by Neis.telid go nonhoeae @A. up to 2070 ofwomen become slerile aRer one episode-ofdiseaseB. patients often exhibit high fever ard abdomiml pain rC. intauteire devices for birth control can increase the incidence of PID. Di s)anptoms of PID are caused by a toxic shock syndrome toxinE. 10% to 20% of