COURSE OUTLINE Course Number: BIO208 Credits: 4 Course Title: GENETICS Hours: 3 lecture/3 laboratory Required texts: iGenetics: A Molecular Approach, 3rd ed. Peter J. Russell Benjamin Cummings Publishers www.geneticsplace.com Course Coordinator: Laura Blinderman Office: MS 110 Phone: 570-3833 [email protected] www.mccc.edu/~blinderl Catalogue Description: A survey course examining gene activity at the molecular and organismal levels. Principles of transmission, molecular, and population genetics are covered with emphases placed on advances in genetic technology and applications. The laboratory exercises address topics in heredity, chromosome structure, recombinant DNA, bioinformatics, and other molecular biology techniques. Three hours of lecture and one three hour laboratory per week. Prerequisites: Successful completion of BIO 102 (C grade or higher) or consent of instructor2 Course Competencies/Goals 1. Elucidate the structure, packaging, and regulation of DNA 2. Analyze the architecture of eukaryotic and prokaryotic genes and describe the regulation of gene expression at the molecular level 3. Explore transmission genetics and solve problems in the transmission of traits 4. Investigate chromosomes, sex linkage, karyotypes, and aneuploidy 5. Examine microbial genetics, mechanisms of gene transfer, and the use of microbes as model organisms 6. Explore techniques and goals of genomics, biotechnology, transgenics, and cloning 7. Elucidate the molecular mechanisms of DNA mutation and repair 8. Investigate topics in population and evolutionary genetics 9. Develop skills in pipetting, gene cloning, bacterial transformation, restriction enzyme digestion, DNA fingerprinting, PCR, bioinformatics, gel electrophoresis, DNA and protein purification, spectrophotometry, centrifugation, and other laboratory techniques Specific objectives by unit will be distributed in class Grading Lecture: The lecture grade is based on examination grades, in-class activities, and homework assignments. The instructor must be informed within 12 hours of a missed exam. Makeup exams, given only for a valid and documented absence, are discouraged, and consist of essay questions. Laboratory: The laboratory grade is based on lab practicals, pre-labs, laboratory work, attendance, and participation. The instructor will evaluate student performance throughout the semester. More than one unexcused absence will result in a lower lab grade. There are no makeup laboratories. Lab work 35% Lecture exams 55% Lecture homework , worksheets Presentation and/or paper 10% Classroom conduct The college welcomes students into an environment that creates a sense of community pride and respect. Attendance It is a student’s responsibility to attend all classes. If a class meeting is missed, the student is responsible for content covered, announcements made in his/her absence, and for acquiring any materials distributed in class. More than 3 missed lectures may result in a lower grade for the course. The laboratory component of the course is critical3 to satisfying the course objectives. Missed laboratories and associated assignments cannot be made up. A student who misses more than two unexcused laboratory sessions will fail the course. A passing grade must be obtained in the laboratory in order to pass the course. Tardiness It is expected that students will be on time for all classes. Students late for an exam may be denied the opportunity to take the exam. A student who enters the laboratory late may not be able to participate in the lab. Behavior Students are expected to follow ordinary rules of courtesy during class sessions. The instructor has the right to eject a disruptive student from the class at any time. Cell phones and other devices are to be turned off prior to the start of class and are not to be used during the class or laboratory session. Text messaging during class is not acceptable. Accommodations A student who has special needs because of a disability is entitled to receive accommodations. Eligible students are assured services under the Americans with Disabilities Act and Section 504 of the Rehabilitation Act of 1973. Contact Arlene Stinson, Director of Academic Support Services, LB221, 570-3525. Academic Integrity Statement: Cheating of any kind is not tolerated. Cheating includes copying papers or website information, presenting another person's work as one's own in any way, looking at a student's paper during a test or quiz, looking at notes during an exam or quiz, obtaining information about an exam, quiz, or any other information that other students do not have and the instructor does not intend them to have, or talking during an exam or quiz. Write your own papers using your own words. All violations of academic integrity will be reported to the Academic Integrity Committee. For additional information: Refer to the MCCC Student Handbook.4 Schedule of Lecture Topics WEEK TOPIC CHAPTERS I Introduction and DNA Structure 1, 2 II Transcription and Translation 5 III Gene Expression 6 IV (continued) EXAM 1 V Mendelian Genetics and Extensions of Mendel 11, 13, 4 VI Chromosomal Basis of Inheritance and Gene Linkage 12 VII Chromosome Variations 16 EXAM 2 VIII Bacterial Genetics 18 X (continued) 19 XI Recombinant DNA Technology 8, 9 XII (continued) EXAM 3 XIII DNA mutation, Repair, Cancer 7, 20 XIV Applications of Recombinant DNA Technology 9, 10 XV Population and Evolutionary Genetics 21, 23 EXAM 4 Laboratory Schedule Lab Topic 1 Lab notebook, Bioinformatics (computer lab) 2 Isolation of DNA from animal and/or plant tissue 3 UV spectrophotometry 4 DNA fingerprinting via the polymerase chain reaction (PCR) 5 Electrophoresis 6 Mendelian Genetics, Chi Square, Probability, Pedigree analysis 7 Cytogenetics, Karyotyping 8 Restriction enzyme mapping 9 DNA mapping (continued) 10 Drosophila pigment chromatography 11 Bioinformatics 12 Gene Expression: Transformation of bacteria with pGLO plasmid 13 Transformation analysis 14 Human evolutionary and population genetics (computer lab) *The Laboratory Schedule may be revised THE COURSE INSTRUCTOR RESERVES THE RIGHT TO CHANGE THE SCHEDULE AND GRADING PROCEDURE AT ANY TIME5 LABORATORY SAFETY Your instructor will call attention to safety precautions. FOOD AND DRINKS ARE NOT ALLOWED IN THE LABORATORY, ever! Be professional