21 Cards in this Set
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Cell Theory
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a. All living creatures are made of one or more cells.
b. The cell is the structural unit of life.
c. On the earth today, cells only arise from division of pre-existing cells.
d. Earlier versions of Cell Theory thought that living cells could arise from non-living
material.
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Evidence of RNA probs evolved b4 DNA & proteins 2 serve IMP FXNS nd prebiotic world?
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c. RNA can both code for genetic information and act as a catalyst.
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What characteristics distinguish prokaryotic and eukaryotic cells?
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a. Eukaryotes have membrane-bound organelles; prokaryotes do not.
b. Prokaryotes have relatively little DNA; eukaryotes generally have much more.
c. Eukaryotic chromosomes are linear; prokaryotic chromosomes are circular.
d. Prokaryotes possess a unique flagellum.
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Alpha helix formation
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Secondary
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A mutation in the DNA that changes amino acid sequence always alters this
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Primary
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A region of a protein that confers a specific function such as phospholipid binding
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Protein domain
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The crystal structure of a protein
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Tertiary
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Method which antibodies are conjugated 2 fluorochrome, used 2 determine location in cell of specific protein
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immunofluorescence
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False?
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a. Both prokaryotes and eukaryotes can form multicellular organisms.
b. Different cell types in a multicellular organism express different genes.
c. Multicellular organisms can be much more complex than single cell organisms.
d. Single-celled organisms are in general less complex ce…
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Evo relationships btwn groups of organisms = best determined usN which type of info?
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a. Comparison of nucleotide sequences.
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Which of the following is an example of an epigenetic phenomenon?
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b. Methylation of the DNA associated with a gene resulting in the transcription of that gene being inhibited over multiple cell generations.
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13. What is defined as the ability to see two neighboring points in a field as distinct entities?
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c. resolution
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Which conditions would result in the worst resolution in a light microscope?
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d. Red light and 0.7 N. A.
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Which of the following things can limit resolution in microscopy?
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b. The wavelength of illumination light.
c. The numerical aperture of the objective.
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16. You wish to measure calcium concentration changes in living cells. Which of the following microscopic techniques do you think would be most useful?
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c. Fluorescence microscopy using a calcium-sensitive indicator dye that is able to cross a living cell's plasma membrane.
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17. Which statement about light microscopy is true?
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a. A light microscope can generate contrast using interference of out of phase light.
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Absorbance of invisible, UV light by compound & d subsequent release by d compound of some of d energy as longer, visible light wavelengths
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fluorescence
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light scope does molecule absorb energy from light n glow w/ bright color or colors against dark backgrd
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d. fluorescence microscopy
The method is often used to localize specific
molecules within a cell.
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b. short, longer
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In a fluorescence microscope, ______-wavelength incident light is absorbed by the specimen
and reemitted at a _______ wavelength.
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wont be able to see it cuz two object at 50nm...needs to add up to 200nm
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21. The resolution limit of a typical fluorescent microscope is ~200 nm. If two ribosomes that are
50 nm in diameter and 500 nm apart are labeled with a fluorescent antibody, what will you see?
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22. Which of the following is true of fluorescence microscopy?
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e. To view rounded, thicker organisms, a confocal microscope will yield the best
image
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MCB 2210: EXAM 1