Spring 2020 Discussion 11 Exam 3 Preparation MCB 150 1 Probes are prepared to conduct FISH on the promoter region directly upstream of functional copies of human 45S genes These probes should result in region s of fluorescence BL4 DL2 LO 21 1 22 1 A B C D E 2 5 10 280 840 2 An experiment is conducted in which replication in early S phase is monitored with an orange fluorescent label and replication in late S phase is monitored with a blue fluorescent label IF DNA replication was not organized into foci what results would most likely be obtained BL4 DL3 LO 21 3 A A uniform diffuse orange color would be detected primarily at the periphery of the nucleus and a uniform diffuse blue color would be detected primarily in the interior of the nucleus B Discrete orange spots would be detected primarily in the interior of the nucleus and a uniform diffuse blue color would be detected primarily at the periphery of the nucleus C A uniform diffuse brown color would be detected throughout the nucleus D Discrete blue spots would be detected primarily at the periphery of the nucleus and a uniform diffuse orange color would be detected primarily in the interior of the nucleus E A uniform diffuse blue color would be detected primarily at the periphery of the nucleus and a uniform diffuse orange color would be detected primarily in the interior of the nucleus 3 Which of the following is an example of a ribonucleoprotein complex BL5 DL2 LO 22 1 26 2 Student Submitted snoRNPs A B SRP C Ribosomes A and C D E A B and C 1 Spring 2020 Discussion 11 Exam 3 Preparation MCB 150 4 You are performing a pulse chase experiment to monitor a set of proteins known to be directly involved in the binding of mRNA molecules to facilitate the export of those mRNAs Which one of the following figures represents the most likely results from your experiment at a time point of two hours after the chase In each of the figures below the large circle represents a cell and the 8 black dots are representative of the set of proteins labeled during the pulse BL4 DL3 LO 25 2 26 1 5 cell Golgi bodies BL5 DL3 LO 28 2 are processed in cell Golgi bodies but not Photosynthetic enzymes plant animal A B Cell wall components plant animal C Acid hydrolases animal plant D E B and C A B and C 2 Spring 2020 Discussion 11 Exam 3 Preparation MCB 150 6 Consider the following two statements about the nucleus 1 The human nucleus is organized into approximately 300 replicons per focus 2 Nuclear speckles are most commonly found in proximity of origins of of replication replication Determine which one of the statements below is correct BL5 DL2 LO 21 2 Student Submitted A Statement 1 and statement 2 are both true B Statement 1 and statement 2 are both false C Statement 1 is true statement 2 is false D Statement 1 is false statement 2 is true 3 Spring 2020 Discussion 11 Exam 3 Preparation MCB 150 Use the following figure to answer the next two 2 questions In this figure EM stands for Extracellular Matrix and CY stands for Cytoplasm Only a general attempt has been made to draw structures to scale 7 If the vesicle labeled 10 is headed from the region labeled 7 to the compartment labeled 15 that vesicle is most likely transporting BL4 DL2 LO 29 2 plasma membrane receptor molecules A B ER translocon channel proteins C D E acid hydrolases secreted proteins proton pump components 4 Spring 2020 Discussion 11 Exam 3 Preparation MCB 150 8 Using greater than less than or approximately equal to identify the correct relationship among the labeled compartments with respect to proton concentration within them Consider pH differences of 0 1 or less approximately equal BL4 DL2 LO 29 2 A B C D E 13 15 17 18 13 15 17 18 13 15 17 18 13 15 17 18 13 15 17 18 5