Discussion 10 Signaling MCB 150 Directions Fill out the table involving transport to various cellular locations The beginning and ending locations have been provided Starting point Ending Point Signal s presented Nuclear Localization Signal NLS Nuclear Export Signal NES Amino terminal signal Internal signal sequence stop transfer sequence Transported via vesicles Mannose 6 phosphate Cytoplas m Nucleus Nucleus Cytoplasm Cytoplas m ER RER Golgi Golgi Lysosome Cytoplas Mitochondr m ia Location of translation Cytoplasm for example Type of molecule amino acid lipid nucleic acid etc Amino acid Location and or arrangement of signal more specific than the previous column Key characteristi c of the signal Anywhere on the protein can also be bipartite Rich in basic amino acids Cytoplasm Amino acid Anywhere on the protein RER membrane Amino acid Terminal of protein near the N terminal of protein RER RER Carbohydrates Mannose 6 phosphate Proteins bud off in vesicles to be transported to the cis face of the golgi apparatus 6th mannose of 11 mer Examples Signaling molecules carbohydrates and lipids into the nucleus Rna ribosomal subunits Proteins bound for golgi apparatus endosomes lysosomes Proteins in the golgi secretory pathway Acid hydrolases Rich in leucine Terminal of protein near the N terminal of protein Long branched chains of sugar Sent out via vesiclea N terminal signal Cytoplasm Amino acid N terminal of protein Terminal of the protein Mitochondrial proteins
View Full Document
Unlocking...