Exam 2 sample questions True-False. ___ The only known DNA double helical structure is called B-DNA and is the one discovered by Watson and Crick.___ DNA replication requires a type of RNA polymerase.___ Each eukaryotic chromosome must contain the following DNA sequence elements: multiple origins of replication, two telomeres, and one centromere. ___ Telomeres are made of repeating DNA sequences. ___ Nucleosome particles are 30nm in diameter and when lined up one after the other (beads-on-a-string), form 30-nm fibers.Eukaryotic RNA polymerases (like RNA polymerase II) are regulated by sigma factors.___ A nucleosome contains two molecules each of histones H2A and H2B as well two molecules each of histones H3 and H4. ___ Eukaryotic RNA polymerases (like RNA polymerase II) are regulated by sigma factors.Multiple choice - Choose the correct answer . ___ Which of the following are not part of Chargaff’s rules?a. Moles purines= moles pyrimidinesb. Moles A = moles Tc. Moles G = moles Cd. The DNA double helix is anti-parallele. All of the above ___ DNA isolated from cow liver contains 14% A. What percent will be T?1a. 14%b. 22%c. 28%d. 36%e. 56%.___ Origins of DNA replication can be visualized by an experimental technique that involvesa. labeling cells with radioactive thymidine and carrying out autoradiography.b. labeling cells with radioactive methionine and carrying out autoradiography.c. labeling cells with radioactive cystine and carrying out autoradiographyd. labeling cells with radioactive carbon and carrying out autoradiography. ___ Which statement(s) are correctly describe properties of Telomerasea. synthesizes telomeres at the ends of the chromosomeb. is required for continued cell divisionc. tends to be expressed at low levels in non-dividing cellsd. uses an internal RNA temple to synthesize the telomere repeate. all of the above ___ The 5'-3' linkage of nucleotides in nucleic acids occurs througha. glycosidic bondsb. peptide bondsc. phosphodiester bondsd. hydrogen bondse. disulfide bonds ___ mRNA is synthesized by:a. RNA Polymerase Ib. DNA Polymerasec. RNA Polymerase IId. Ligasee. none of the above ___ The transcriptional regulation of the tryptophan operon occurs through2a. the binding of a transcriptional activator protein.b. the binding of TFIIDc. the binding of a transcriptional repressor that is regulated in its binding to DNA.d. phosphorylation of the sigma factor Proteins bound to centromere sequences form a complex known as the ________________.Upon incorporation of histone H1 into chromatin, the nucleosomes coil into a structure called the __________________________.Define the following terms b. Chromatinc. E.coli sigma factorsd. TATA boxe. Histone 3Below is a segment of RNA from the middle of a mRNA. 5’-UGGUCUAGGCACGGA-3’This segment of RNA is part of the coding region of an mRNA for a protein.Give the amino acid sequence for the protein that is encoded by this segment of mRNA.