BCHM 4116 1st Edition Lecture 14 Outline of Last Lecture I DNA replication Outline of Current Lecture I DNA replication Current Lecture 1 How is DNA Replicated a Semiconservative i 1 of 2 original strands are conserved in each of the 2 progeny b Bidirectional i Begins at 1 or more specific region called origin of replication ii Involves 2 replication forks that move in opposite directions c Semi discontinuous i Continuous strand Leading strand ii Discontinuous strand Lagging strand via Okazaki fragments iii d Replication i DNA gyrase 1 Type II topoisomerase 2 Overcome torsional stress imposed upon unwinding by introducing negative supercoils ii Helicase 1 Unwinds 2 Disrupt hydrogen bonds iii Single stranded DNA binding proteins 1 Binds to single strands to prevent from re annealing iv DNA Polymerase 1 Uses ssDNA as template 2 Synthesize DNA only in 5 3 v DNA Pol I 1 Only Pol with 5 3 exonuclease meaning it can replace RNA primer with DNA vi DNA ligase 1 Seals remaining nicks in Okazaki fragments vii Ter Region 1 DNA replication terminates Ter These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute 2 Termination requires binding of Tusprotein toTer a Tus is a counterhelicase i Tus prevents DNA duplex from unwinding by blocking progression of replication fork
View Full Document
Unlocking...