UCSD BICD 110 - REVIEW PROBLEMS (3 pages)

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REVIEW PROBLEMS



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REVIEW PROBLEMS

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Problems/Exams


Pages:
3
School:
University of California, San Diego
Course:
Bicd 110 - Cell Biology
Cell Biology Documents

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KEY FOR BICD 110 REVIEW PROBLEMS I For each of the following molecules indicate WHERE they could be found in the cell a LDL receptor plasma MB endocytic vesicle MB recycling endosme MB all membranes between PM endoxome lysosome b Signal Recognition Particle Receptor ER MB c Ran GTP predominantly nucleus also some in cytoplasm d Sar1 GTP ER MB e Clathrin on surface of certain vesicles TGN to late endosome endocytic vesicle TGN to secretory vesicles f GPI anchored protein plasma MB or in Golgi where made then in vesicles while being transported to surface g Na glucose symporter plasma MB of cells or in compartment where made h Adaptins in clathrin coated vesicles associated with clathrin i COPII on surface of vesicles ER to Golgi k GlcNAc phosphotransferase Golgi l KDEL receptor in Golgi ER or vesicles between the two compartments II Short answer questions a Which of the following organisms would have the highest percentage of unsaturated fatty acid chains in their membranes i ii iii dog bacterium that lives in thermal vent in Yellowstone arctic fish b Name two lipids that tend to be concentrated in lipid rafts Cholesterol sphingolipids glycolipids c Name one cellular process that involves lipid rafts Targeting to apical MB d If you removed the signal sequence from protein disulfide isomerase where would the protein end up cytoplasm e How is it that multiple ER signal sequences can be recognized by a single protein SRP has methionie rich hydrophobic pocket that can recognize and bind multiple hydrophobic sequences f An early experiment demonstrated that membranes were likely made of a bilayer of lipids What type of cells were used for these experiments Red blood cells g Why was this particular type of cell chosen in part h they have no organelle membranes just plasma membranes h Jeopardy answer FRAP Fluorescence Recovery after photobleaching You name the question i e pose a question that would have FRAP as the correct answer What technique could you use to measure the



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