CChheemm 44334433 Quiz Nov 2010 Name : Answer each of the following five parts for a total of 100 points. 1. (i) The process below represents the enzymatic catalysis of the glycosidic bond, that permits the incorporation of isotopic oxygen (labeled as 18O below) into one of the products. Provide the mechanism for this reaction making sure to draw the isotope-labelled product and draw an appropriate resonance structure for the cation produced in the first step : (ii) Both enzymes shown to the right catalyze similar substrates. Show the initial mechanism for both the Serine Protease and the HIV Protease and identify any differences between the optimum pH for each process.(iii) dentify, giving your reasoning, an appropriate pH rangefor the enzymatic reaction shown on the right and comment on the role of the Zn2+ present in the active site. (iv) For the normal, i.e. wild type (WT), enzyme, mutant enzyme (M), normal substrate (S) and inhibitor (I), i.e. drug shown on the right : Identify the forces responsible for formation of the EWTS and EMDrug complexes Draw the Lineweaver-Burk plots for EWT+S , EWT+S+I and EM+S+I (v) The structures below show the substrate and product of an enzymatic reaction that catalyzes the oxidation of an aldehyde to carboxylic acid. Given the kinetic assay data shown calculate KM and Vmax for this substrate (ignore units) and identify, giving your reasoning, what each line in the plot
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