ST. EDWARDS CHEM 4343 - Study Notes (6 pages)

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Study Notes



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Study Notes

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6
School:
St. Edward's University
Course:
Chem 4343 - Biochemistry I
Biochemistry I Documents

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Chem 4343 Final Exam 2009 Name Answer any seven of questions 1 8 Each question is worth 15 points for a total of 105 points 1 i For the HIV protease enzyme the natural substrate and two drugs are shown on the far right along with the Val residue in the binding pocket Given the kinetic data on the right identify giving your reasoning which line belongs to which drug and calculate KM for the reaction with normal substrate ii For the enzymatic reaction on the right draw the mechanism and suggest specific amino acids for A and B in the diagram above iii Lysozyme is a small enzyme which hydrolyzes polysaccharide chains using amino acids Glu35 and Asp52 as shown below Identify giving your reasoning the optimum pH range for this reaction and comment on the nature of the ES complex that is formed 2 i Identify giving your reasoning an appropriate pH range for the enzymatic reaction shown on the right and comment on the role of the Zn2 present in the active site ii Both enzymes shown to the right initiate catalysis by attack in the substrate Show the initial mechanism for both the Serine Protease and the HIV Protease and identify any differences between the optimum pH for each process iii In the hydrolysis reaction shown below the same substrate is shown bound to three different enzymes the normal or wild type enzyme wt and two mutants that have one amino acid changed in the active site Determine giving your reasoning which column of kinetic data belongs to which mutant and calculate KM and Vmax for the wild type enzymatic assay S mM 0 5 1 2 10 20 50 75 V wt S s 33 50 67 91 95 98 99 V S s 11 20 33 71 83 93 95 V S s 17 25 33 45 48 49 49 3 i Line up one of the RNA nucleotides below in the box on the right in such a way as to give the best h bonded interaction between the two bases ii For the DNA micrograph image on the right what level of structure does this represent and what function s does the above structure serve in the organism iii The positively charged histones shown on



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