BIOL 4352 Exam 1 Study Guide Lectures 1 9 Lecture 2 August 26 An introduction What is the primary job function of a crime scene investigator This section or agency or entity is responsible for the recognition documentation collection preservation and transportation of the evidence Which of the following are examples of sections that are typically in a state or police agency s crime laboratory Forensic Biology Controlled substances Forensic Investigations etc are typical sections that are present in a state agency s crime laboratory Which type of physical evidence collection method is typically used to collect biological material Swabbings cuttings and whole item are all examples of evidence collection methods Lecture 3 August 28 Which phage of hair growth would you most likely obtain the most amount of viable biological material for DNA testing The Anagen phase would be the first phase of the growth cycle with the most amount of DNA because the catagen phase would have disturbed or inactive hair growth and the telogen phase would be the end of the growth cycle with the lease amount of DNA present Suppose you were able to collect biological evidence at very specific layers and portions of a tooth which layer portion of the tooth would you expect not be able to collect biological material for DNA testing from a tooth that has been knocked out with a punch during an assault and aggravated robbery The dentin and enamel layers both would have no cells present Suppose you were able to collect biological evidence at very specific layers and portions of a tooth which layer portion of the tooth would you expect to be able to collect biological material for DNA testing from a tooth that has been knocked out with a punch during an assault and aggravated robbery The pulp cavity and root canal both house the nerves and blood vessels which would contain biological materials present Which of the following does not contribute to the fluorescence of semen under ALS Lipids do not contribute to semen fluorescence What are examples of things that contribute to the fluorescence of semen under ALS Flavins and proteins containing Amino Acids with aromatic 6 carbon rings can contribute to the florescence of semen under ALS Which of the following blood components would get you DNA if you were able to separate each of them out Plasma white blood cells and red blood cells can possibly get you DNA What does presumptive testing rely on Proteins Lecture 3 September 2 What is the purpose of conducting a crime scene investigation The purpose of conducting a crime scene investigation is to secure the scene document and preserve physical evidence It also responds to an initiated criminal investigation by law enforcement It also ensures that the collected preserved biological evidence can be safely transported to a laboratory for subsequent analysis while maintaining its integrity Who or what gives you the authority to conduct a criminal investigation Your lab manager gives you the authority Prior to collecting biological evidence at a crime scene what is required to collect such biological evidence An evidentiary search warrant is required Lecture 4 September 4 Which of the following would be best to collect a very small bloodstain on the hood of a car Swabbing would be the best method to collect a very small bloodstain on the hood of a car What is a proper seal And what are some characteristics of a proper seal It maintains the integrity of the evidence It contains date and initials It tamper proofs the evidence and must touch both sides of the evidence container s opening Since evidence can be submitted in four ways a wet bloody shirt can be Submitted by Certified mail after air drying True or false In the FTA method the DNA can be trapped within the paper True Lecture 5 September 16 True or false An antiglobulin recognizes the antigen in the immunochromatographic assays False An antibody recognizes the antigen in an immunochromatographic assay Which of the following dictates directs or influences the crime laboratory processes policies And or procedures how the crime laboratory operates and conducts forensic biology Testing Lecture 6 September 18 The police department the National Academy of Sciences NAS the Laboratory manager The FBA DNA Advisory board and the ISO Certification Entity all contribute in some way What is a presumptive test It categorizes similar class structures Which of the following would be a comfirmatory test most crime laboratories are not currently using Teichman Heme microcrystal test and the Takayama Hemochromagen microcrystal Test are tests that crime laboratories rarely use Which of the following involves the use of specific wavelengths of light for reflection Absorption and or emission for visualization The ALS Fluorescine Chemiluminescence assays and Flavins What test would be the closest to a confirmatory test The Immunochromatographic assay tests Lecture 7 September 30 Why would you only want to use a portion of the evidence in Serology You would only want to use a portion of the evidence in serology to be able to retest For defense testing DNA testing and archiving What is an example of a primary binding assay ELISA and Immunochromatographic assays are examples of primary binding assays What is the most sensitive assay that could be used for detection of a specific antigen like PSA ELISA is the most sensitive assay for detection of specific antigens What is true about presumptive tests Presumptive tests involve false positives that exist and are performed Prior to confirmatory testing It is quick and easy and helps streamline DNA testing Lecture 8 October 2 Which are some factors that could negatively affect blood evidence and its Subsequent presumptive testing Some factors include oxidation and humidity of the testing location What can successful DNA testing not be performed on Successful DNA can not be performed on adult red blood cells and platelets Which statement is not true about hemoglobin What is not true about hemoglobin is that it contains two polypeptides 4 different polypeptide Chain types and is responsible for the transport of CO2 Instead it contains four polypeptides and is responsible for two different polypeptide chains and is also for the transport of O 2 Which of the following statements is true about the basis of presumptive tests for blood Oxidative reduction reaction requires a catalyst it is a colorimetric assay and hydrogen Peroxide is converted to water