BIOL 4352 Exam 2 Study Guide Lectures 10 15 Lecture 10 October 9 How long can AP still be viable in the vaginal vault after death It can still be viable for up to 7 days in the vagina Why use AP for presumptive testing when it can be found in other bodily fluids and materials Seminal activity is about 1000 times greater than other bodily fluids such as vaginal fluids A semen stain was revealed upon examining evidence on a pant leg what is the best way to test for Acid Phosphatase The best way to test for Acid Phosphatase is with a moistened swab rub the stain What wavelength of light from an ALS would you use to visualize a semen stain when wearing Orange goggles 455 Nm Lecture 11 October 21 Nucleotides in linear polynucleotides DNA chains are linked together by what kind of chemical bonds 5 3 Phosphodiester bonds Which statement is false The Short arm is designated as P because it is the point of attachment Nearly 60 of the human genome consists of intergenic non coding sequences in current forensics PCR based DNA analysis relies upon which type of coding sequence STR s Current DNA testing relies upon length polymorphisms that are called alleles to create a genotype for a region of DNA which allows forensic scientist to use a collection of these to generate a DNA profile True Lecture 12 October 23 Which of the following contains Deoxyribose a Nitrogenous base and a Phosphate group DNA and Nucleotide What are characteristics of Cytosine and thymine They are nucleobases nucleotides pyrimidines and nucleosides DNA s double helix is stabilized with what kind of interactions Chemical interactions and hydrogen bonds Lecture 13 October 28th What are the main types of DNA extractions that are used by crime laboratories PCIA Chelex Silica Based and FTA Under what conditions does DNA bind to silica resin It binds to silica resin under high salt and chaotrophic buffer What degrades proteins when using a lysis buffer Protease detergents reducing agents buffer s pH above 8 and proK How is the bound DNA released from the silica resin It is bound by the elusion buffer and the charge switch Lecture 14 October 30th Which DNA extraction method will provide the most cleanest and highest molecular weight DNA in solution PCIA What would you expect to find in the aqueous phase top layer DNA Using the DNA IQ DNA Extraction Kit how would you obtain a final concentration of DNA at 0 5 ng ul by changing what component s It adjusts both the Elusion buffer and the resin Which of the DNA extraction methods can you obtain large amounts of ssDNA from 5 Chelex solution What does the Chelex 100 resin sequester during heating at 56 degrees Celsius Divalent cations ions The DNA probe D17Z1 used for Slot Blots is specific for Humans and primates Why should we or do we in the forensics field perform DNA Quantitation To see if we have PCR inhibitors to save money in downstream analyses To predict expect how efficient our DNA sample will amplify for DNA analysis The PCR based quantification methods are Human and primate specific Lecture 15 October 4th If you had to perform a DNA quantitation method but did not have the time resources or ability to create a standard curve from known DNA concentrations which DNA quantitation method would you use Tube based quantification method Reverse transcriptase PCR utilizes which kind of primers for the generation of cDNA DNA primers Real Time PCR can monitor the PCR efficiency during amplification by using Lag exponential linear and plateau phases Fluorescence detection and S Shaped curve PCR efficiency can be affected specifically and or generally by Low amounts of input template DNA Low Copy Number Amplification Inhibitors associated with forensic samples Inhibitors from DNA extraction components and the cycling parameters What are characteristics of mRNA oligonucleotides They have 5 3 phosphodiester bonds the mRNA oligonucleotides can undergo PCR for the identification of biological sources such as tissues or bodily fluids and they are unstable