SC BIOL 541 - Catalysis Mechanism (4 pages)

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Catalysis Mechanism

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Catalysis Mechanism


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Lecture Note
University Of South Carolina-Columbia
Biol 541 - Biochemistry

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BIOL 541 1st Edition Lecture 11 Outline of Last Lecture I Co enzymes and co factors Outline of Current Lecture II Molecular mechanisms of catalysis III Six major groups of enzymes Current Lecture Biochem Biol 541 Lecture 11 Glomerulization of enzymes metabolon 3 examples of molecular mechanisms of catalysis Lysozyme This is found in tears and egg whites It is anti bacterial in nature and digests bacterial cell wall It has specificity for polysaccharide that alternates between acetyl glucosamine and OH that replaces the hetero polysaccharide 2 sugars forms NAG and NAM It is a relatively small protein of 129 amino acids and a molecular weight of 14 600 Da It is single chain globular in structure and stabilized by intra chain disulphide bonds It has an overall cleft which is the substrate binding site places for sugars Amino acids from lysozyme can make contact with the polysaccharide chains There are non covalent bonds that decrease the free energy all except those for binding of 4 th sugar Chain fits into cleft D sugar is in the chair conformation i e stressful and high energy conformation It is not favorable except it decreases free energy from 5th residue in order to drive the reaction therefore termed as energy leaver There is also reduced fit allosterism Try 62 moves in 1 A upon substrate binding Cleavage occurs at chair sugar and cleavage proceeds via lactone derivative that forms chair chair is intermediate structure active sites and catalysis Inactive site protonated GLU acid and ionized ASP acid occurs at pk3 However local environment increases pk3 value so that both can be protonated But in tears since These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute extracellular fluid is acidic has pH 5 0 GLU acid has a value more than that and ASP has a value less than that Therefore GLU acid is protonated and ASP acid is ionized H from the GLU acid acts as acid and

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