Biol 423L Laboratories in Genetics 1 Biology 423L Syllabus 2011 Laboratory Experiments in Genetics Lecture Monday at 12 00 noon in Wilson Hall 128 Laboratories Section 001 Tuesday at 2 00 p m in Wilson room 242 Section 002 Wednesday at 2 00 p m in Wilson room 242 Course materials available on Blackboard Text book Hartwell et al 4th ed I strongly recommend buying the text but one copy will be on the text book cart at the reserve circulation desk in the undergraduate library available for two hours at a time I will also note pages in the syllabus below for the 3rd edition of the same text book which is also available at the reserve desk There is no course pack All lab protocols reading lists and most reading materials will be available on blackboard A new version of the protocol will be available one week before each lab Print out the protocol read it carefully and bring it to class Instructor Sarah Grant Email sgrant email unc edu Telephone 919 962 0684 Office hours by appointment email me if you want to set up a meeting Friday afternoons are optimal TA Email Office hours by appointment TA Email Office hours to be announced Reports Due in laboratory period 2 weeks after exercise finished Penalty for handing in late 50 off if handed in by 5 00 Wednesday for Section 001 and Thursday for section 002 Otherwise your report will not be graded and your grade will be recorded as 0 points Exceptions can be made in unusual circumstances by arrangement email to TA or instructor before due date Reports will be handed back one week after being turned in Lab reports will make up 50 of the final grade They will be graded out of 25 points 5 for participation 5 for abstract and introduction 10 for methods and results and 5 for discussion See Course Information on web site for instructions on how to prepare lab reports Exams etc 1 midterm for 15 of final grade Biol 423L Laboratories in Genetics 2 1 research paper 10 of final grade Final exam 25 of final grade Syllabus Date Topic August 23 24 No labs classes and labs will start next week August 29 First Lecture Mendelian Genetics Readings for class Genes and alleles Hartwell Chapter 2 pp 13 27 Chi square test Hartwell Chapter 5 pp 127 130 Complementation Hartwell Chapter 7 pp 225 226 Aug 30 31 First lab exercise Mendelian Genetics using Fast Plants Report on Mendelian Genetics due Sept 13 14 Sept 5 Labor Day no lecture Sept 6 7 Pipetteman and Titration Exercise Alleles in Yeast You will need to come back on day 2 after the lab to check results Report on Yeast Alleles due in lab Sept 20 21 Sept 12 Mouse Models and Epistasis research paper goals Readings for class Hartwell Chapter 3 pp 56 71 For discussion in class Bowman et al 1991 Development 112 1 20 PDF available on Blackboard Sept 13 14 Epistasis using Mice Report on Epistasis in Mice due Sept 27 28 in lab period Report on Mendelian Genetics due in lab this week Start thinking about research paper topics Sept 19 Yeast as a Model System and mutation Reading Mutagenesis Hartwell Chapter 7 pp 208 224 PDFs on Blackboard The Response of Yeast to Radiation A Closer Look at Biological Consequences of UV exposure A Closer Look at Repair of DNA Sept 20 21 Mutation in Yeast You will need to come back on day 3 after the lab to check results Plant Arabidopsis seeds Report on Mutation in Yeast due Oct 4 5 in lab period Report on Yeast Alleles due in lab this week Sept 26 Bacterial Genetics Class Biol 423L Laboratories in Genetics 3 Readings for class Bacterial Genetics Hartwell Chapter 15 pp 543 562 For discussion in class Lederberg and Tatum 1946 J Bact 53 673684 PDF available on Blackboard Sept 27 28 Conjugation Lab You will need to come back on day 3 after the lab to check results Report on Conjugation Lab due Oct 11 12 Report on Epistasis in Mice due this week Oct 3 C elegans as a Model System and Epigenetics Readings for class C elegans Hartwell Chapter 8 pp 255 256 Epigenetics Hartwell Chapter 18 pp 665 668 726 728 Tabara et al 1999 Cell 99 123 132 Verdel et al 2009 Int J Dev Biol 53 245 257 Eymery et al 2009 Int J Dev Biol 53 259 268 Oct 4 5 and 11 12 C elegans Genetics and RNAi You will need to come back on day 4 day 8 and day 12 after the first lab to continue experiments This will involve one hour on one weekend day the second week Times for all returns will be organized by the TA during the lab periods Report on C elegans Genetics and RNAi due Oct 25 26 Report on Mutation in Yeast due this week Oct 4 5 Oct 10 Epigenetics Oct 11 12 Continue C elegans Genetics and RNAi lab Research paper topics due Oct 15 send email to Dr Grant by 5 pm Oct 15 Report on C elegans Lab due Oct 25 26 Report on Conjugation Lab due this week Oct 17 Midterm Oct 18 19 Nov 1 2 Nov 15 16 Oct 18 19 Drosophila three point cross Transplant Arabidopsis Report on Drosophila three point cross due Nov 20 30 Oct 24 Three point cross and mapping in humans Readings for class Drosophila Hartwell Chapter 20 pp 732 749 Sex linked inheritance Hartwell Chapter 4 pp 105 112 Three point cross Hartwell Chapter 5 pp 123 141 Map based cloning of the gene for Cystic Fibrosis Readings for class Cystic fibrosis Hartwell Chapter 5 pp 142 143 Map based cloning in humans Hartwell Chapter 11 pp 408 419 For discussion in class Pearson 2009 Nature 460 165 169 Oct 25 26 DNA Cloning lab Run gels set up ligations and transformations Biol 423L Laboratories in Genetics 4 You will need to come back on day 2 after the lab for about 15 minutes work and on day 3 to check results Readings Hartwell Chapter 9 pp 303 326 1 2 page outlines for research papers due October 29 email to Dr Grant by 5 pm Report on DNA Cloning Lab due Nov 8 9 Report on C elegans Lab due in lab this week Oct 31 Drosophila as a Model System Guest lecturer Dr Bob Duronio Nov 1 2 Drosophila Test Cross Score for sex linkage or autosomal markers continue 3 point cross Nov 7 DNA Markers and Forensics Readings for class PCR and forensics Hartwell Chapter 9 pp 327 330 For discussion in class Lander E S and J J Ellis 1998 Nature 396 13 14 Forster et al 1998 1998 Nature 396 13 14 Nov 8 9 DNA Markers and Forensics lab Reading Nakamura et al 1988 NAR 16 9364 Report on DNA Cloning lab due this week Nov 14 Map Based Cloning in Model Organisms Readings for class Physical Mapping of genes Hartwell Chapter 10 pp 354 365 Lukowitz et al 2000 Plant Physiol …