Biol 423L Laboratories in Genetics 1 Biology 423L Syllabus 2009 Laboratory Experiments in Genetics Lecture Monday at 11 00 am in Wilson Hall 128 Laboratories Web site Section 001 Tuesday at 2 00 p m in Wilson room 242 Section 002 Wednesday at 2 00 p m in Wilson room 242 http www bio unc edu courses 2009Fall Biol423L Text book Hartwell et al ed 3 I strongly recommend buying the text but one copy will be on reserve for 2 hours at a time in the Undergraduate library There is no course pack All lab protocols reading lists and most reading materials will be available from the web site A new version of the protocol will be available one week before each lab Print out the protocol read it carefully and bring it to class Instructor Sarah Grant Email sgrant email unc edu Telephone 919 962 0684 Office hours Thursdays from 4 00 5 00 in Coker 213A TA Vanessa Gonzalez Perez Email Vanessa gonzalez med unc edu Office hours To be announced TA Louise Giffin Email giffin email unc edu Office hours To be announced Reports Due in laboratory period 2 weeks after exercise finished Penalty for handing in late 50 off if handed in by 5 00 Wednesday for Section 001 and Thursday for section 002 Otherwise your report will not be graded and your grade will be recorded as 0 points Exceptions can be made in unusual circumstances by arrangement email to TA or instructor before due date Reports will be handed back one week after being turned in Lab reports will make up 50 of the final grade They will be graded out of 25 points 5 for participation 5 for abstract and introduction 10 for methods and results and 5 for discussion See Course Information on web site for instructions on how to prepare lab reports Exams etc 1 midterm for 15 of final grade 1 research paper 10 of final grade Final exam 25 of final grade Biol 423L Laboratories in Genetics 2 Syllabus Date Topic August 25 26 No labs classes and labs will start next week August 31 First Lecture Mendelian Genetics Readings for class Genes and alleles Hartwell Chapter 2 pp 13 27 Complementation Hartwell Chapter 7 pp 225 226 Sept 1 2 First lab exercise Mendelian Genetics using Fast Plants Report on Mendelian genetics due Sept 8 9 Sept 7 Labor Day no lecture Sept 8 9 Pipetteman and Titration Exercise Alleles in Yeast Report on Yeast Alleles due in lab Sept 15 16 Report on Mendelian Genetics using Fast Plants due in lab this week Sept 14 Mouse Models and Epistasis Readings for class Hartwell Chapter 3 pp 56 71 For discussion in class Bowman et al 1991 Development 112 1 20 PDF available on web site Sept 15 16 Epistasis using Mice Report on Epistasis in Mice due Sept 29 30 in lab period Report on Yeast Alleles due in lab this week Sept 21 Yeast as a Model sSystem guest lecture Dr Elaine Yeh Reading Mutagenesis Hartwell Chapter 7 pp 208 224 PDFs on web site The Response of Yeast to Radiation A Closer Look at Biological Consequences of UV exposure A Closer Look at Repair of DNA Sept 22 23 Mutation in Yeast Report on Mutation in Yeast due Oct 6 7 in lab period Report on Epistasis in Mice due in lab this week Sept 28 Bacterial Genetics Class Readings for class Bacterial Genetics Hartwell Chapter 15 pp 543 562 For discussion in class Lederberg and Tatum 1946 J Bact 53 673684 PDF available on web site Sept 29 30 Conjugation Lab Report on Conjugation Lab due Oct 13 14 Report on Epistasis in Mice due this week Biol 423L Laboratories in Genetics 3 Oct 5 C elegans as a Model System and Epigenetics Readings for class C elegans Hartwell Chapter 8 pp 255 256 Epigenetics Hartwell Chapter 18 pp 665 668 726 728 Tabara et al 1999 Cell 99 123 132 Verdel et al 2009 Int J Dev Biol 53 245 257 Eymery et al 2009 Int J Dev Biol 53 259 268 Oct 6 7 and 13 14C elegans Genetics and RNAi Report on C elegans Genetics and RNAi due Oct 20 21 Report on Mutation in Yeast due this week Oct 12 University Day class cancelled Oct 13 14 Continue C elegans Genetics and RNAi lab Research paper topics due Oct 14 send email to Dr Grant by 5 pm Oct 14 Report on C elegans Lab due Oct 27 28 Report on Conjugation Lab due this week Oct 19 Three point cross and mapping in humans Readings for class Drosophila Hartwell Chapter 20 pp 732 749 Sex linked inheritance Hartwell Chapter 4 pp 105 112 Three point cross Hartwell Chapter 5 pp 123 141 Map based cloning of the gene for Cystic Fibrosis Readings for class Cystic fibrosis Hartwell Chapter 5 pp 142 143 Map based cloning in humans Hartwell Chapter 11 pp 408 419 For discussion in class Pearson 2009 Nature 460 165 169 Oct 20 21 Nov 3 4 Nov 17 18 Drosophila three point cross Report on Drosophila three point cross due Dec 1 2 Oct 26 Midterm Oct 27 28 DNA Cloning lab Run gels set up ligations and transformations Readings Hartwell Chapter 9 pp 303 326 Report on DNA Cloning Lab due Nov 10 11 Report on C elegans Lab due in lab this week Nov 2 Drosophila as a Model System Guest lecturer Dr Bob Duronio Nov 3 4 Drosophila Test Cross Score for sex linkage or autosomal markers continue 3 point cross 1 2 page outlines for research papers due November 6 email to Dr Grant by 5 pm Biol 423L Laboratories in Genetics 4 Nov 9 DNA Markers and Forensics Readings for class PCR and forensics Hartwell Chapter 9 pp 327 330 For discussion in class Lander E S and J J Ellis 1998 Nature 396 13 14 Forster et al 1998 1998 Nature 396 13 14 Nov 10 11 DNA Markers and Forensics lab Report on DNA Cloning lab due this week Nov 16 Map Based Cloning in Model Organisms Readings for class Physical Mapping of genes Hartwell Chapter 10 pp 354 365 Lukowitz et al 2000 Plant Physiol 123 795 805 Nov 17 18 Score 3 pt Cross Report on Drosophila and 3 pt Cross due Dec 1 2 Nov 23 Association mapping for human diseases Reading for class Hartwell Chapter 11 pp 423 425 Research papers due in class Monday Nov 23 No lab this week Thanksgiving Nov 30 Map Based Cloning continues Dec 1 2 Map Based Cloning lab Report on Drosophila lab due this week Dec 2 3 Dec 7 Results of Map Based Cloning Review Dec 14 exam 12 00 noon to 3 00 pm Final Exam 128 Wilson Hall