BIO 183 Exam 3 Fall 2018 Please read the instructions below carefully then fill out the Scantron sheet as requested You may write on the examination but only the scantron will be used to determine your grade for the multiple choice questions When you are finished with the exam hand in both the scantron and the written exam to your instructor proctor For the Scantron part of the exam mark answers in the appropriate bubbles Please follow the instructions for use of the scantron sheet exactly Failure to follow these instructions carefully will result in a penalty of 1 point on the exam You should begin by filling in only the following information on the Scantron sheet items must be both written in the spaces available and marked in the appropriate bubbles Your name last name followed by one space followed by your first name Your student ID if available You may not use any supporting material calculators phones computers on the written part of the exam you must answer the questions in the space provided Only questions answered in non erasable pen will be eligible for a re grade Honor code I PRINT sign my name to this Honor Code to declare my intentions of upholding the ideals of academic honesty as a student at North Carolina State University I understand that plagiarism and other forms of cheating specified in Student Law are unconscionable misconduct subject to review by campus courts I have neither given nor received unauthorized aid in completing this examination Signature My lab section is Day Time Multiple Choice Choose the best answer for each Write your answers on the provided Scantron sheet 2 points each 1 Sister chromatids move toward the opposite poles of a dividing cell during a Telophase of mitosis b Anaphase of meiosis I c Prophase of mitosis d Prophase of meiosis I and II e Anaphase of meiosis II a Metaphase I b Anaphase I c Prophase I II d Anaphase II e Metaphase II a Cleavage furrow b A septum c Cytokinetic constriction d A cell plate e Mitotic split 2 In what phase of meiosis do homologous chromosomes line up in the middle of the cell 3 The membrane that forms at the end of telophase to separate daughter cells at the end of mitosis in plants is known as 4 Which of the following is NOT a reason for mitotic cell division a Pollen production in flowers b Fetal growth c Tissue repair after injury d Tumor formation e All of the above are reasons for mitotic cell division 5 Which of the following is are true about nucleosomes a Nucleosomes are arranged in a zig zag pattern to make a 30nm DNA fiber b Nucleosomes contribute greatly to DNA compaction c Nucleosomes may be found in euchromatin or heterochromatin d Nucleosomes are found in eukaryotes e All the above are true 6 Nucleosomes are composed of DNA and proteins known as a SNPs b Kinetochores c Centrioles d Histones 7 DNA follows a pattern of replication known as a Conservative b Conservative Semi conservative or dispersive depending on the type of cell c Liberal d Dispersive e Semi conservative 8 Cyclin dependent kinases CDKs are required for which of the following processes a DNA replication during S phase b DNA transcription c Cell cycle regulation check point regulation d Sister chromatids separation during mitosis e Cellular communication 9 In the karyotype of a carrot cell you would expect to see a More chromosomes than in a human karyotype b Fewer chromosomes than in a human karyotype c The same number of chromosomes as in a human karyotype since carrots are diploid too d It depends on the stage of the cell cycle during which the comparison is made e There is no way to predict an organism s chromosome count just by looking at its phenotype 10 The protein complexes that hold sister chromatids at their center are known as 11 Typically in a healthy cell the longest phase of the cell cycle is the 12 Which of the following proteins acts as an important tumor suppressor a Centrioles b Cohesins c Kinetochores d Spliceosomes e Centromeres a M phase b G1 phase c G2 phase d S phase e Cytokinesis a SNP b Ras c P53 d MPF e Gyrase A Phosphatase B Catalase C Hydroxylase D Kinase E Phosphate dehydrogenase A ATP conservation B Conservation of energy C Signal amplification and speed D Signal redundancy E Enzyme recycling 13 What type of enzyme is usually associated with the transfer of phosphate groups from high energy phosphate donating molecules to specific substrates i e associated with the phosphorylation of substrates 14 The advantage of using Ca2 cAMP or a pyramid shaped cascade of events downstream of membrane bound receptors is Short Anwers 1 Epinephrine is a ligand that binds to adrenergic receptors on cardiac muscle cells adrenergic receptors are a type of G protein coupled receptors GPCRs In the space below CLEARLY draw a cell membrane with an embedded adrenergic receptors and label the various components of your drawing In a few words explain the steps that follow the activation of this receptor by epinephrine Be as thorough and specific as possible I do not want you to draw the entire mechanism Just draw and explain the first few steps leading to phospholipase C or adenylyl cyclase activation 10 points Molecules that bind to GPCRs would most likely be large and hydrophilic small and hydrophobic circle the correct answer The cellular response to this type of stimulus would most likely happen in 1 second 1 day circle the correct answer 2 The structure of a DNA or RNA nucleotide may be simplified as follows P CH 2 O In the space provided below link 2 of these nucleotides as if they were part of the same DNA strand Then clearly indicate the 3 and the 5 ends of your strand The bond formed between nucleotides in the sugar phosphate backbone of DNA and mRNA is called a bond while the bonds that link the 2 strands to each other between purines and pyrimidines are called bonds 8 points 3 In mammals females are born with all the primary oocytes gametes located in ovaries that they will ever release At birth the primary oocytes are arrested in prophase I and during each round of the estrous or menstrual cycle a few primary oocytes proceed to develop into secondary oocytes that are arrested in metaphase II until fertilization takes place In the space below draw a primary oocyte for a diploid mammal that has a 2N 4 as it develops into a secondary oocyte i e as it goes from prophase I to Metaphase II Your drawing should at least include the chromosomes chromatids centrosomes centromeres membranes etc Clearly label the various