Name ONLINE Transformation Post Lab Assignment Post Lab Assignment Students will be expected to complete this assignment after reading watching the associated content for this assignment in the Online Week 1 folder on eLC This post lab assignment will require you to understand the concepts and importance of genetic transformation gene regulation and the techniques pathways through which they are achieved This assignment should be completed individually in adherence to the UGA Academic Honesty Policy Use of sources outside of material posted in eLC or the lab manual is not all should convey student understanding of the concepts and content presented and represent their original thought and written work All responses owed Please submit your PDF to the eLC drop box by 9 00 P M Sunday April 5 2020 Bacterial transformation concepts 1 In your own words what is genetic transformation 1 pt Genetic transformation is a process in which DNA of an organism is changed by inserting a foreign gene into the cell 2 How is genetic transformation beneficial to an organism in its natural environment 1 pt Genetic transformation helps to make a modified version of the organism which is more adaptive to its surrounding 3 What is a plasmid 1 pt Plasmid is a circular DNA which is present in bacteria 4 What characteristics make plasmids ideal for performing transformations 1 pt Plasmids are very small thus it can carry the gene easily from one organism to another Plasmids have multiple binding sites which means the plasmid can be cut and a section of a foreign gene can be pasted on the binding site 5 Why is bacteria an ideal model organism for transformation experiments 1 pt Bacteria is an ideal model organism for transformation because only bacteria have plasmids and ribosomes which is ideal for DNA replication and production ONLINE Transformation Post Lab Assignment Transformation experiment 1 What feature of the pGLO plasmid s expression will identify a successful transformation 1 pt pGLO act as gene marker It is initially inserted into the plasmid to observe the transformation process If the end product of the sample shows a color by shining on fluorescent light then it can be confirmed that it was a successful transformation 2 What bacterial growth pattern would you expect on each of the four agar plates above and why 4 pts A less pattern will be seen than others because it does not have arabinose B More growth pattern will be seen than others because it has arabinose than increases production of pGLO C No pattern will be seen as there is no gene marker D No pattern will be seen as there is no amp ara and also no gene markers 3 Which agar plate s serve as your positive control How do es the plate s serve this role 3 pts B serves as positive control This is because 4 Which agar plate s serve as your negative control How do es the plate s serve this role 3 pts D serves as negative control This is because it does not have amp or ara as well as no pGLO thus it can be compared with others ONLINE Transformation Post Lab Assignment Transformation Efficiency Use the following equation and sample plate to determine the sample efficiency of the trial g To find the number of cells growing you must count the number of glowing colonies present on the LB amp ara plate shown below The amount of DNA spread on the plate refers to the total amount of pGLO plasmid introduced during the experiment Assume that the experiment was done according to the posted Transformation Protocol Hint 10 l of the DNA plasmid was used and the plasmid was provided at a concentration of 0 08 g l Calculated transformation efficiency 1 pt Would you consider this to be a successful experiment Why or why not 3 pts Yes I will consider this as a successful experiment because Transformation efficiency is greater than 50