Identifying Bacteria and Viruses - Methods for Identifying Bacteriao Morphology Better suited for eukaryoteso Differential staining Gram stains Acid-fast stainso Biochemical tests Tests for the presence of certain enzymes o Molecular Genetics Tests PCR, rRNA sequencing, RFLP- Bergey’s Manualso Determinate Bacteriology Arranged by phenotypes Emphasis on identification o Systematic Bacteriology Focused on phylogeny More in-depth - Dichotomous Keyso Flow chart to rapidly eliminate options for microbe identificationo Asks easy yes or no questions Example: Is this microbe gram positive? - Classifying Viruseso Viruses are acellular Requires own classification o Classified by shape and genetic code RNA vs DNA Single stranded vs double stranded + or – RNA strando Grouped into families Example: orthomyxovirus o Grouped into species Example: influenzao Grouped into strains Example: influenza Ao Grouped into subtypes Example: influenza A
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